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RAB7A / RAB7 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249664

RAB7A Antibody, CMT2B Antibody, PRO2706 Antibody, RAB7 Antibody, Ras-associated protein RAB7 Antibody, Ras-related protein Rab-7a Antibody, PSN Antibody Description: Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation,… More details

$650 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAB7A / RAB7, Polyclonal Antibody
Full Product Name
Anti-RAB7A / RAB7 Antibody (C-Terminus) IHC-plus
Product Gene Name
anti-RAB7A / RAB7 antibody
Product Synonym Names
RAB7A; CMT2B; PRO2706; RAB7; Ras-associated protein RAB7; Ras-related protein Rab-7a; PSN
Clonality
Polyclonal
Isotype
IgG
Host
RAB7A/RAB7 antibody was produced in Goat
Species Reactivity
Human, Monkey, Mouse, Rat , Dog
Form/Format
PBS, 0.05% Sodium Azide, 20% Glycerol
Purity/Purification
Immunoaffinity purified
Concentration
3 mg/mL (lot specific)
Specificity
Detects Rab7a protein in the human, rat and mouse whole cell lysates and transfected cells with GFP-Rab7a by Western blot. This Ab is specific for Rab7a.
Preparation and Storage
Short term: Store at 4°C ; Long term: Store at -20°C. Avoid freeze/thaw cycles.
OMIM
600882
3D Structure
ModBase 3D Structure for P51149
Immunogen
RAB7A / RAB7 antibody was raised against purified recombinant peptide derived from within residues 110 aa of the C-terminus of mouse Rab7a produced in E Coli.
Immunogen Species
RAB7A/RAB7 antibody was raised against Mouse.
Antigen Type
Recombinant protein
Epitope
C-terminus
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-RAB7A / RAB7 antibody
Immunohistochemistry (IHC) - Paraffin, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-RAB7A / RAB7 antibody
IHC- Paraffin (5 ug/mL)
Immunofluorescence (1:50-1:250)
Western Blot (1:250-1:2000)

Optimal dilution to be determined by the researcher.

Other Notes

Small volumes of anti-RAB7A / RAB7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAB7A / RAB7 purchase

MBS249664 is an antibody targeting RAB7A / RAB7. Also known as Anti-RAB7A / RAB7 Antibody (C-Terminus) IHC-plus. Reactive in Human, Monkey, Mouse, Rat , Dog. Host: RAB7A/RAB7 antibody was produced in Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for RAB7A / RAB7. It may not necessarily be applicable to this product.

NCBI GI #
1709999
NCBI GeneID
7879
NCBI Accession #
P51149.1
UniProt Primary Accession #
P51149
UniProt Secondary Accession #
Q9NWJ0; Q9UPB0; A8K3V6
UniProt Related Accession #
P51149
NCBI Official Full Name
Ras-related protein Rab-7a
NCBI Official Synonym Full Names
RAB7A, member RAS oncogene family
NCBI Official Symbol
RAB7A
NCBI Official Synonym Symbols
RAB7; PRO2706
UniProt Protein Name
Ras-related protein Rab-7a
UniProt Gene Name
RAB7A
UniProt Synonym Gene Names
RAB7
UniProt Entry Name
RAB7A_HUMAN
NCBI Protein Information
ras-related protein Rab-7a

NCBI Summary for RAB7A / RAB7

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]
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UniProt Comments for RAB7A / RAB7

Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.

Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; late endosome; late endosome membrane; lipid particle; lysosomal membrane; lysosome; melanosome membrane; phagocytic vesicle; phagocytic vesicle membrane; pre-autophagosomal structure membrane; retromer complex; terminal button; vacuolar membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding; Rac GTPase binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; bone resorption; early endosome to late endosome transport; endocytosis; endosome to lysosome transport; epidermal growth factor ligand processing; positive regulation of protein catabolic process; positive regulation of viral reproduction; protein targeting to lysosome; protein to membrane docking; protein transport; release of virus from host; retrograde transport, endosome to Golgi; small GTPase mediated signal transduction

Disease: Charcot-marie-tooth Disease, Axonal, Type 2b
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Research Articles on RAB7A / RAB7

1. Hepatitis B virus can regulate its own secretion through an activation of the endo-lysosomal and autophagic pathway mediated by Rab7 activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAB7A / RAB7 antibody

 Reference Product  PubMed Publications
 RAB11A antibody  >3 publications with RAB7A / RAB7 and RAB11A
 RAB1A antibody  >1 publications with RAB7A / RAB7 and RAB1A
 RAB11B antibody  >1 publications with RAB7A / RAB7 and RAB11B
 VPS41 antibody  >1 publications with RAB7A / RAB7 and VPS41
 VPS39 antibody  >1 publications with RAB7A / RAB7 and VPS39

Pathways associated with anti-RAB7A / RAB7 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 MHC Class II Antigen Presentation Pathway antibodies  MHC Class II Antigen Presentation Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram

Diseases associated with anti-RAB7A / RAB7 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with RAB7A / RAB7 and Nervous System Diseases
 Liver Diseases Antibodies  >3 publications with RAB7A / RAB7 and Liver Diseases
 Peripheral Nervous System Diseases Antibodies  >2 publications with RAB7A / RAB7 and Peripheral Nervous System Diseases
 Charcot-Marie-Tooth disease, Type 2B Antibodies  >2 publications with RAB7A / RAB7 and Charcot-Marie-Tooth disease, Type 2B
 Fever Antibodies  >1 publications with RAB7A / RAB7 and Fever
 Inflammation Antibodies  >1 publications with RAB7A / RAB7 and Inflammation
 Kidney Diseases Antibodies  >1 publications with RAB7A / RAB7 and Kidney Diseases
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