• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Choline Acetyltransferase/CHAT (CHAT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752969

Description: Rabbit IgG polyclonal antibody for Choline Acetyltransferase/CHAT detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.Background: Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Choline Acetyltransferase/CHAT, Polyclonal Antibody
Full Product Name
Anti-Choline Acetyltransferase/CHAT Antibody
Product Gene Name
anti-CHAT antibody
Product Synonym Names
Choline O-acetyltransferase (EC:2.3.1.6); CHOACTase; ChAT; Choline acetylase; CHAT
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
gene 254210
3D Structure
ModBase 3D Structure for P28329
Immunogen
E Coli-derived human Choline Acetyltransferase/CHAT recombinant protein (Position: T25-K731).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CHAT antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-CHAT antibody
WB: 0.25-0.5ug/ml (Human, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Flow Cytometry (FC/FACS) of anti-CHAT antibody

Rabbit Anti-CHAT Polyclonal Antibody – Cat# MBS1752969

Immunofluorescence (IF) of anti-CHAT antibody

Rabbit Anti-CHAT Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1752969

Immunohistochemistry (IHC) of anti-CHAT antibody

Rabbit Anti-CHAT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1752969

Western Blot (WB) of anti-CHAT antibody

Figure 1. Western blot analysis of CHAT using anti-CHAT antibody.
Rabbit Anti-CHAT Polyclonal Antibody, Western Blot (WB) – Cat# MBS1752969

Product References and Citations for anti-CHAT antibody

1. Cohen-Haguenauer, O., Brice, A., Berrard, S., Van Cong, N., Mallet, J., Frezal, J. Localization of the choline acetyltransferase (CHAT) gene to human chromosome 10. Genomics 6: 374-378, 1990.
2. Harold, D., Peirce, T., Moskvina, V., Myers, A., Jones, S., Hollingworth, P., Moore, P., Lovestone, S., Powell, J., Foy, C., Archer, N., Walter, S., and 11 others. Sequence variation in the CHAT locus shows no association with late-onset Alzheimer's disease. Hum. Genet. 113: 258-267, 2003.
3. Kraner, S, Laufenberg, I., Strassburg, H. M., Sieb, J. P., Steinlein, O. K. Congenital myasthenic syndrome with episodic apnea in patients homozygous for a CHAT missense mutation. Arch. Neurol. 60: 761-763, 2003.

Other Notes

Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHAT purchase

MBS1752969 is an antibody targeting CHAT. Also known as Anti-Choline Acetyltransferase/CHAT Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.

NCBI GI #
281185509
NCBI GeneID
1103
NCBI Accession #
P28329.4
UniProt Primary Accession #
P28329
UniProt Related Accession #
P28329
NCBI Official Full Name
Choline O-acetyltransferase
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
UniProt Protein Name
Choline O-acetyltransferase
Protein Family
(Z)-3-hexen-1-ol acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
UniProt Entry Name
CLAT_HUMAN
NCBI Protein Information
choline O-acetyltransferase

NCBI Summary for CHAT

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Show more

UniProt Comments for CHAT

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: EC 2.3.1.6; Lipid Metabolism - glycerophospholipid; Acetyltransferase

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cell soma; mitochondrion; axon; cytoplasm; nucleus; cytosol

Molecular Function: choline O-acetyltransferase activity

Biological Process: rhythmic excitation; muscle development; neurotransmitter secretion; glycerophospholipid biosynthetic process; adult walking behavior; synaptic transmission; rhythmic behavior; phospholipid metabolic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; neurotransmitter biosynthetic process

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
Show more

Research Articles on CHAT

1. The SNP rs3753841 in COL11A1, rs1258267 in CHAT and rs736893 in GLIS3 are associated with PACG in northern Chinese people, and the association of genetic markers manifests a tendency of ethnic diversity. Larger population-based studies are warranted to reveal additional primary angle-closure glaucoma loci and ethnic aspects of primary angle-closure glaucoma.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHAT antibody

 Reference Product  PubMed Publications
 ACHE antibody  >884 publications with CHAT and ACHE
 NGF antibody  >594 publications with CHAT and NGF
 SLC18A3 antibody  >130 publications with CHAT and SLC18A3
 SLC5A7 antibody  >8 publications with CHAT and SLC5A7
 VAMP2 antibody  >1 publications with CHAT and VAMP2
 KIFAP3 antibody  >1 publications with CHAT and KIFAP3

Pathways associated with anti-CHAT antibody

 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram

Diseases associated with anti-CHAT antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2517 publications with CHAT and Nervous System Diseases
 Brain Diseases Antibodies  >1351 publications with CHAT and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1037 publications with CHAT and Neurodegenerative Diseases
 Neurobehavioral Manifestations Antibodies  >888 publications with CHAT and Neurobehavioral Manifestations
 Disease Models, Animal Antibodies  >552 publications with CHAT and Disease Models, Animal
 Nerve Degeneration Antibodies  >436 publications with CHAT and Nerve Degeneration
 Movement Disorders Antibodies  >272 publications with CHAT and Movement Disorders
 Memory Disorders Antibodies  >266 publications with CHAT and Memory Disorders
 Cognition Disorders Antibodies  >249 publications with CHAT and Cognition Disorders
 Learning Disorders Antibodies  >224 publications with CHAT and Learning Disorders

Organs/Tissues associated with anti-CHAT antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5902 publications with CHAT and Brain
 Eye Antibodies  >553 publications with CHAT and Eye
 Testis Antibodies  >13 publications with CHAT and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000