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RAPGEF3 / EPAC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249823

RAPGEF3 Antibody, Bcm910 Antibody, CAMP-GEFI Antibody, CGEF1 Antibody, HSU79275 Antibody, EPAC Antibody, EPAC 1 Antibody, EPAC1 Antibody Description: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP.… More details

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Product Overview

Product Name
RAPGEF3 / EPAC, Polyclonal Antibody
Full Product Name
Anti-RAPGEF3 / EPAC Antibody (N-Terminus) IHC-plus
Product Gene Name
anti-RAPGEF3 / EPAC antibody
Product Synonym Names
Rabbit Polyclonal to Human RAPGEF3 / EPAC; Human RAPGEF3 / EPAC; Bcm910; CAMP-GEFI; CGEF1; HSU79275; EPAC 1; EPAC1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat, Human
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity purified
Concentration
1 mg/ml (lot specific)
Specificity
Human RAPGEF3 / EPAC. At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.
Preparation and Storage
Short term 4 degree C
Long term aliquot and store at -20 degree C
Avoid freeze thaw cycles. Store undiluted.
Molecular Weight
99,452 Da
OMIM
606057
3D Structure
ModBase 3D Structure for O95398
Immunogen
RAPGEF3 / EPAC antibody was raised against an 18 amino acid peptide near the amino terminus of human EPAC1
Immunogen Description
An 18 amino acid peptide near the amino terminus of human EPAC1
Immunogen Type
Synthetic peptide
Antigen Modification
N-Terminus
Target Species
Human
Applications Tested/Suitable for anti-RAPGEF3 / EPAC antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RAPGEF3 / EPAC antibody
IF (20 ug/ml)
IHC-P (5 ug/ml)
WB (1 ug/ml)

Immunohistochemistry (IHC) of anti-RAPGEF3 / EPAC antibody

Anti-RAPGEF3 / EPAC antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RAPGEF3 / EPAC Polyclonal Antibody – Cat# MBS249823

Immunofluorescence (IF) of anti-RAPGEF3 / EPAC antibody

Immunofluorescence of EPAC1 in rat brain tissue with EPAC1 antibody at 20 µg/mL.
Rabbit Anti-RAPGEF3 / EPAC Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS249823

Western Blot (WB) of anti-RAPGEF3 / EPAC antibody

Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 ug/ml.
Rabbit Anti-RAPGEF3 / EPAC Polyclonal Antibody, Western Blot (WB) – Cat# MBS249823

Other Notes

Small volumes of anti-RAPGEF3 / EPAC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAPGEF3 / EPAC purchase

MBS249823 is an antibody targeting RAPGEF3 / EPAC. Also known as Anti-RAPGEF3 / EPAC Antibody (N-Terminus) IHC-plus. Reactive in Mouse, Rat, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAPGEF3 / EPAC. It may not necessarily be applicable to this product.

NCBI GI #
20070215
NCBI GeneID
10411
NCBI Accession #
NP_006096.2
UniProt Primary Accession #
O95398
UniProt Secondary Accession #
O95634; Q8WVN0; A8K2G5; E7EQC8
UniProt Related Accession #
O95398
GenBank Nucleotide #
NM_006105.5
NCBI Official Full Name
rap guanine nucleotide exchange factor 3 isoform b
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor 3
NCBI Official Symbol
RAPGEF3
NCBI Official Synonym Symbols
EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
UniProt Protein Name
Rap guanine nucleotide exchange factor 3
UniProt Synonym Protein Names
Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI
UniProt Gene Name
RAPGEF3
UniProt Synonym Gene Names
CGEF1; EPAC; EPAC1; EPAC 1; cAMP-GEFI
UniProt Entry Name
RPGF3_HUMAN
NCBI Protein Information
rap guanine nucleotide exchange factor 3

UniProt Comments for RAPGEF3 / EPAC

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, misc.; GEFs

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: apical part of cell; basal plasma membrane; brush border membrane; cell soma; cortical actin cytoskeleton; endomembrane system; filopodium; growth cone; lamellipodium; membrane; microvillus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: cAMP binding; guanyl-nucleotide exchange factor activity; protein binding; protein domain specific binding; Rap guanyl-nucleotide exchange factor activity

Biological Process: angiogenesis; blood coagulation; brain development; cAMP-mediated signaling; cell proliferation; energy reserve metabolic process; negative regulation of collagen biosynthetic process; platelet activation; positive regulation of angiogenesis; positive regulation of cAMP catabolic process; positive regulation of cell size; positive regulation of GTPase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; positive regulation of smooth muscle cell migration; positive regulation of stress fiber formation; positive regulation of syncytium formation by plasma membrane fusion; positive regulation of transcription from RNA polymerase II promoter; Rap protein signal transduction; regulation of insulin secretion; response to nicotine; signal transduction; spinal cord development
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Research Articles on RAPGEF3 / EPAC

1. Identified a conserved nuclear pore localisation signal (NPLS; amino acids 764-838 of EPAC1) in the catalytic domains of the cAMP-sensors, EPAC1 and EPAC2A. EPAC1 and EPAC2, display distinct subcellular distributions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAPGEF3 / EPAC antibody

 Reference Product  PubMed Publications
 RAP1A antibody  >7 publications with RAPGEF3 / EPAC and RAP1A
 RAP1B antibody  >6 publications with RAPGEF3 / EPAC and RAP1B
 PDE3B antibody  >3 publications with RAPGEF3 / EPAC and PDE3B
 RAP1GAP antibody  >3 publications with RAPGEF3 / EPAC and RAP1GAP
 PDE4A antibody  >1 publications with RAPGEF3 / EPAC and PDE4A
 ABCC8 antibody  >1 publications with RAPGEF3 / EPAC and ABCC8

Pathways associated with anti-RAPGEF3 / EPAC antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway antibodies  Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Integrin AlphaIIb Beta3 Signaling Pathway antibodies  Integrin AlphaIIb Beta3 Signaling Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-RAPGEF3 / EPAC antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >16 publications with RAPGEF3 / EPAC and Inflammation
 Cardiovascular Diseases Antibodies  >15 publications with RAPGEF3 / EPAC and Cardiovascular Diseases
 Hypertrophy Antibodies  >14 publications with RAPGEF3 / EPAC and Hypertrophy
 Heart Diseases Antibodies  >13 publications with RAPGEF3 / EPAC and Heart Diseases
 Lung Diseases Antibodies  >7 publications with RAPGEF3 / EPAC and Lung Diseases
 Heart Failure Antibodies  >6 publications with RAPGEF3 / EPAC and Heart Failure
 Edema Antibodies  >5 publications with RAPGEF3 / EPAC and Edema
 Necrosis Antibodies  >5 publications with RAPGEF3 / EPAC and Necrosis
 Disease Models, Animal Antibodies  >5 publications with RAPGEF3 / EPAC and Disease Models, Animal
 Fibrosis Antibodies  >4 publications with RAPGEF3 / EPAC and Fibrosis

Organs/Tissues associated with anti-RAPGEF3 / EPAC antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >42 publications with RAPGEF3 / EPAC and Vascular
 Blood Antibodies  >36 publications with RAPGEF3 / EPAC and Blood
 Muscle Antibodies  >34 publications with RAPGEF3 / EPAC and Muscle
 Heart Antibodies  >31 publications with RAPGEF3 / EPAC and Heart
 Lung Antibodies  >24 publications with RAPGEF3 / EPAC and Lung
 Brain Antibodies  >15 publications with RAPGEF3 / EPAC and Brain
 Pancreas Antibodies  >13 publications with RAPGEF3 / EPAC and Pancreas
 Kidney Antibodies  >9 publications with RAPGEF3 / EPAC and Kidney
 Umbilical Cord Antibodies  >6 publications with RAPGEF3 / EPAC and Umbilical Cord
 Prostate Antibodies  >6 publications with RAPGEF3 / EPAC and Prostate
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