• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ARHGEF12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249871

ARHGEF12 Antibody, KIAA0382 Antibody, LARG Antibody, PRO2792 Antibody, Leukemia-associated RhoGEF Antibody Description: May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
ARHGEF12, Polyclonal Antibody
Full Product Name
Anti-ARHGEF12 Antibody (aa435-484) IHC-plus
Product Gene Name
anti-ARHGEF12 antibody
Product Synonym Names
Rabbit Polyclonal (IgG) to Human ARHGEF12; Human ARHGEF12; KIAA0382; LARG; PRO2792; Leukemia-associated RhoGEF
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat, Human
Form/Format
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity purified
Concentration
1 mg/ml (lot specific)
Specificity
ARHGEF12 Antibodyantibody detects endogenous levels of ARHGEF12.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
171,115 Da
OMIM
604763
3D Structure
ModBase 3D Structure for Q9NZN5
Immunogen
ARHGEF12 antibody was raised against synthetic peptide from human ARHGEF12 (aa435-484).
Immunogen Description
Synthetic peptide from human ARHGEF12 (aa435-484).
Antigen Modification
aa435-484
Target Species
Human
Applications Tested/Suitable for anti-ARHGEF12 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
Application Notes for anti-ARHGEF12 antibody
ELISA (1:10000)
IHC-P (20 ug/ml)
WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-ARHGEF12 antibody

Anti-ARHGEF12 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Rabbit Anti-ARHGEF12 Polyclonal Antibody – Cat# MBS249871

Western Blot (WB) of anti-ARHGEF12 antibody

Western blot of the lysates from HUVEC cells using ARHGEF12 antibody.
Rabbit Anti-ARHGEF12 Polyclonal Antibody, Western Blot (WB) – Cat# MBS249871

Other Notes

Small volumes of anti-ARHGEF12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARHGEF12 purchase

MBS249871 is an antibody targeting ARHGEF12. Also known as Anti-ARHGEF12 Antibody (aa435-484) IHC-plus. Reactive in Mouse, Rat, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGEF12. It may not necessarily be applicable to this product.

NCBI GI #
7662088
NCBI GeneID
23365
NCBI Accession #
NP_056128.1
UniProt Primary Accession #
Q9NZN5
UniProt Secondary Accession #
O15086; Q6P526
UniProt Related Accession #
Q9NZN5
GenBank Nucleotide #
NM_015313.2
NCBI Official Full Name
rho guanine nucleotide exchange factor 12 isoform 1
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 12
NCBI Official Symbol
ARHGEF12
NCBI Official Synonym Symbols
LARG; PRO2792
UniProt Protein Name
Rho guanine nucleotide exchange factor 12
UniProt Synonym Protein Names
Leukemia-associated RhoGEF
Protein Family
Rho guanine nucleotide exchange factor
UniProt Gene Name
ARHGEF12
UniProt Synonym Gene Names
KIAA0382; LARG
UniProt Entry Name
ARHGC_HUMAN
NCBI Protein Information
rho guanine nucleotide exchange factor 12

NCBI Summary for ARHGEF12

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Show more

UniProt Comments for ARHGEF12

ARHGEF12: a widely-expressed guanine nucleotide exchange factor (GEF) for RhoA GTPase. May act as GTPase-activating protein (GAP) for G-alpha 12 and 13. The fusion protein MLL-ARHGEF12 appears to be a disease driver in acute myeloid leukemia. Two isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; Motility/polarity/chemotaxis; Oncoprotein; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; cytosol; membrane

Molecular Function: G-protein-coupled receptor binding; GTPase activator activity; protein binding; Rho guanyl-nucleotide exchange factor activity

Biological Process: axon guidance; G-protein coupled receptor protein signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of GTPase activity; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction
Show more

Research Articles on ARHGEF12

1. this study identified a novel association between IOP and ARHGEF12.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARHGEF12 antibody

 Reference Product  PubMed Publications
 RHOA antibody  >48 publications with ARHGEF12 and RHOA
 CDC42 antibody  >9 publications with ARHGEF12 and CDC42
 PLXNB1 antibody  >3 publications with ARHGEF12 and PLXNB1
 RHOC antibody  >2 publications with ARHGEF12 and RHOC
 RHOB antibody  >1 publications with ARHGEF12 and RHOB

Pathways associated with anti-ARHGEF12 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 G Alpha (12/13) Signalling Events Pathway antibodies  G Alpha (12/13) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 NRAGE Signals Death Through JNK Pathway antibodies  NRAGE Signals Death Through JNK Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram

Diseases associated with anti-ARHGEF12 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >6 publications with ARHGEF12 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >6 publications with ARHGEF12 and Vascular Diseases
 Leukemia, Myeloid, Acute Antibodies  >6 publications with ARHGEF12 and Leukemia, Myeloid, Acute
 Hypertension Antibodies  >4 publications with ARHGEF12 and Hypertension
 Nervous System Diseases Antibodies  >3 publications with ARHGEF12 and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >2 publications with ARHGEF12 and Cell Transformation, Neoplastic
 Heart Diseases Antibodies  >2 publications with ARHGEF12 and Heart Diseases
 Liver Neoplasms Antibodies  >1 publications with ARHGEF12 and Liver Neoplasms
 Inflammation Antibodies  >1 publications with ARHGEF12 and Inflammation
 Lung Diseases Antibodies  >1 publications with ARHGEF12 and Lung Diseases

Organs/Tissues associated with anti-ARHGEF12 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >16 publications with ARHGEF12 and Muscle
 Vascular Antibodies  >14 publications with ARHGEF12 and Vascular
 Blood Antibodies  >9 publications with ARHGEF12 and Blood
 Brain Antibodies  >6 publications with ARHGEF12 and Brain
 Prostate Antibodies  >4 publications with ARHGEF12 and Prostate
 Kidney Antibodies  >4 publications with ARHGEF12 and Kidney
 Heart Antibodies  >4 publications with ARHGEF12 and Heart
 Intestine Antibodies  >3 publications with ARHGEF12 and Intestine
 Lung Antibodies  >3 publications with ARHGEF12 and Lung
 Eye Antibodies  >3 publications with ARHGEF12 and Eye
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000