• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

KDM1B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249936

KDM1B Antibody, AOF1 Antibody, BA204B7.3 Antibody, C6orf193 Antibody, DJ298J15.2 Antibody, LSD2 Antibody Description: Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
KDM1B, Polyclonal Antibody
Full Product Name
Anti-KDM1B Antibody (N-Terminus) IHC-plus
Product Gene Name
anti-KDM1B antibody
Product Synonym Names
Rabbit Polyclonal (IgG) to Human KDM1B; Human KDM1B; AOF1; BA204B7.3; C6orf193; DJ298J15.2; LSD2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Human
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity purified
Concentration
1 mg/ml (lot specific)
Specificity
KDM1B antibody is human, mouse and rat reactive. At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms.
Preparation and Storage
Long term: -20 degree C
Short term: 4 degree C
Avoid repeat freeze-thaw cycles
Molecular Weight
19,428 Da
OMIM
613081
3D Structure
ModBase 3D Structure for Q8NB78
Immunogen
KDM1B antibody was raised against 17 amino acid peptide near the amino terminus of human KDM1B.
Immunogen Description
17 amino acid peptide near the amino terminus of human KDM1B.
Antigen Modification
N-Terminus
Target Species
Human
Applications Tested/Suitable for anti-KDM1B antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
Application Notes for anti-KDM1B antibody
IHC-P (10 ug/ml)
WB (1 - 2 ug/ml)

Immunohistochemistry (IHC) of anti-KDM1B antibody

Anti-KDM1B antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-KDM1B Polyclonal Antibody – Cat# MBS249936

Western Blot (WB) of anti-KDM1B antibody

Western blot analysis of KDM1B in 3T3 cell lysate with KDM1B antibody at 2 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-KDM1B Polyclonal Antibody, Western Blot (WB) – Cat# MBS249936

Other Notes

Small volumes of anti-KDM1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KDM1B purchase

MBS249936 is an antibody targeting KDM1B. Also known as Anti-KDM1B Antibody (N-Terminus) IHC-plus. Reactive in Mouse, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KDM1B. It may not necessarily be applicable to this product.

NCBI GI #
317373434
NCBI GeneID
221656
NCBI Accession #
Q8NB78.3
UniProt Primary Accession #
Q8NB78
UniProt Secondary Accession #
Q5TGV3; Q6AI15; Q6ZUU4; Q8N258; Q96EL7; A2A2C5; A2A2C6
UniProt Related Accession #
Q8NB78
NCBI Official Full Name
Lysine-specific histone demethylase 1B
NCBI Official Synonym Full Names
lysine demethylase 1B
NCBI Official Symbol
KDM1B
NCBI Official Synonym Symbols
AOF1; LSD2; C6orf193; bA204B7.3; dJ298J15.2
UniProt Protein Name
Lysine-specific histone demethylase 1B
UniProt Synonym Protein Names
Flavin-containing amine oxidase domain-containing protein 1; Lysine-specific histone demethylase 2
Protein Family
Lysine-specific histone demethylase
UniProt Gene Name
KDM1B
UniProt Synonym Gene Names
AOF1; C6orf193; LSD2
UniProt Entry Name
KDM1B_HUMAN
NCBI Protein Information
lysine-specific histone demethylase 1B

NCBI Summary for KDM1B

Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009]

UniProt Comments for KDM1B

AOF1: Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Belongs to the flavin monoamine oxidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; FAD binding; zinc ion binding

Biological Process: DNA methylation during gametogenesis; establishment and/or maintenance of chromatin architecture; genetic imprinting; multicellular organismal development; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Show more

Research Articles on KDM1B

1. These results demonstrate an important role for LSD2 in regulation of DNA methylation and gene silencing in breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KDM1B antibody

 Reference Product  PubMed Publications
 HDAC1 antibody  >1 publications with KDM1B and HDAC1
 WHSC1L1 antibody  >1 publications with KDM1B and WHSC1L1
 EHMT2 antibody  >1 publications with KDM1B and EHMT2
 GLYR1 antibody  >1 publications with KDM1B and GLYR1

Pathways associated with anti-KDM1B antibody

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 HDMs Demethylate Histones Pathway antibodies  HDMs Demethylate Histones Pathway Diagram

Diseases associated with anti-KDM1B antibody

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >2 publications with KDM1B and Hypertension
 Breast Neoplasms Antibodies  >1 publications with KDM1B and Breast Neoplasms
 Hyperglycemia Antibodies  >1 publications with KDM1B and Hyperglycemia

Organs/Tissues associated with anti-KDM1B antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with KDM1B and Brain
 Muscle Antibodies  >1 publications with KDM1B and Muscle
 Liver Antibodies  >1 publications with KDM1B and Liver
 Prostate Antibodies  >1 publications with KDM1B and Prostate
 Kidney Antibodies  >1 publications with KDM1B and Kidney
 Heart Antibodies  >1 publications with KDM1B and Heart
 Thyroid Antibodies  >1 publications with KDM1B and Thyroid
 Intestine Antibodies  >1 publications with KDM1B and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000