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PKM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249985

PKM Antibody, p58 Antibody, PK3 Antibody, PKM2 Antibody, Pyruvate Kinase Antibody, Pyruvate kinase 2/3 Antibody, Pyruvate kinase, m1 Antibody, Pyruvate kinase, muscle Antibody, TCB Antibody, Tumor M2-PK Antibody, OIP-3 Antibody, OIP3 Antibody, Opa-interacting… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PKM / Pyruvate Kinase, Polyclonal Antibody
Full Product Name
Anti-PKM / Pyruvate Kinase Antibody (aa383-417) IHC-plus
Product Gene Name
Product Synonym Names
Rabbit Polyclonal to Human PKM / Pyruvate Kinase; Human PKM / Pyruvate Kinase; p58; PK3; PKM2; Pyruvate Kinase; Pyruvate kinase 2/3; Pyruvate kinase; m1; Pyruvate kinase; muscle; TCB; Tumor M2-PK; OIP-3; OIP3; Opa-interacting protein 3; CTHBP; PK; muscle type; PK2; Pyruvate kinase isozymes M1/M2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity purified
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Molecular Weight
56,273 Da
OMIM
179050
3D Structure
ModBase 3D Structure for P14618
Immunogen
PKM / Pyruvate Kinase antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human PKM1.
Immunogen Description
KLH conjugated synthetic peptide selected from the C-terminal region of human PKM1.
Immunogen Type
Synthetic peptide
Antigen Modification
aa383-417
Target Species
Human
Applications Tested/Suitable for anti-PKM antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-PKM antibody
IHC-P (1:100)
WB (1:1000)

Immunohistochemistry (IHC) of anti-PKM antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-PKM Polyclonal Antibody – Cat# MBS249985

Immunohistochemistry (IHC) of anti-PKM antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-PKM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS249985

Western Blot (WB) of anti-PKM antibody

Western blot of PKM1 antibody (C-term L398) in mouse testis tissue lysates (35 ug/lane). PKM1 (arrow) was detected using the purified antibody.
Rabbit Anti-PKM Polyclonal Antibody, Western Blot (WB) – Cat# MBS249985

Western Blot (WB) of anti-PKM antibody

Western blot of PKM1 antibody (C-term L398) in HeLa cell line lysates (35 ug/lane). PKM1 (arrow) was detected using the purified antibody.
Rabbit Anti-PKM Polyclonal Antibody, Western Blot (WB) – Cat# MBS249985

Other Notes

Small volumes of anti-PKM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKM purchase

MBS249985 is an antibody targeting PKM. Also known as Anti-PKM / Pyruvate Kinase Antibody (aa383-417) IHC-plus. Reactive in Mouse, Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PKM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pyruvate kinase PKM isoform a
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
Protein Family
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
NCBI Protein Information
pyruvate kinase PKM

NCBI Summary for PKM

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
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UniProt Comments for PKM

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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