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CAST Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2527359

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CAST, Polyclonal Antibody
Full Product Name
CAST Polyclonal Antibody
Product Gene Name
Product Synonym Names
BS-17
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen affinity purification
Concentration
2.5mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Molecular Weight
82,437 Da
OMIM
114090
Immunogen
Recombinant protein of human CAST
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3

Product Categories/Family for anti-CAST antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CAST antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CAST antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-CAST antibody

Western Blot analysis of Lovo cell using CAST Polyclonal Antibody at dilution of 1:600
Rabbit Anti-CAST Polyclonal Antibody – Cat# MBS2527359

Immunohistochemistry (IHC) of anti-CAST antibody

Immunohistochemistry of paraffin-embedded Human cervical cancer using CAST Polyclonal Antibody at dilution of 1:60
Rabbit Anti-CAST Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2527359

Immunohistochemistry (IHC) of anti-CAST antibody

Immunohistochemistry of paraffin-embedded Human colon cancer using CAST Polyclonal Antibody at dilution of 1:60
Rabbit Anti-CAST Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2527359

Other Notes

Small volumes of anti-CAST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CAST purchase

MBS2527359 is an antibody targeting CAST. Also known as CAST Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CAST. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CAST protein, partial
NCBI Official Synonym Full Names
calpastatin
NCBI Official Symbol
CAST
NCBI Official Synonym Symbols
BS-17
UniProt Protein Name
Calpastatin
UniProt Synonym Protein Names
Calpain inhibitor; Sperm BS-17 component
Protein Family
UniProt Gene Name
CAST
UniProt Entry Name
ICAL_HUMAN
NCBI Protein Information
calpastatin; calpain inhibitor; sperm BS-17 component

UniProt Comments for CAST

calpastatin: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Disease: Peeling Skin With Leukonychia, Acral Punctate Keratoses, Cheilitis, And Knuckle Pads
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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