Rabbit Anti-RFFL Polyclonal Antibody – Cat# MBS2528037

Immunohistochemistry of paraffin-embedded Human liver cancer tissue using RFFL Polyclonal Antibody at dilution 1:25

RFFL Antibody – Rabbit Polyclonal

Name: RFFL Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS2528037
Price: 160 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2528037

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby… More details

$160 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RFFL, Polyclonal Antibody

Full Product Name

RFFL Polyclonal Antibody

Product Gene Name

anti-RFFL antibody

Product Synonym Names

CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Antigen affinity purification

Concentration

1.4mg/mL (lot specific)

Preparation and Storage

Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Molecular Weight

36,633 Da

OMIM

609735

Immunogen

Recombinant protein of human RFFL

Buffer

PBS with 0.05% sodium azide, 50% glycerol, pH7.3

Product Categories/Family for anti-RFFL antibody

Cell Biology

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-RFFL antibody

ELISA (EIA), Immunohistochemistry (IHC)

Application Notes for anti-RFFL antibody

IHC: 1:25-1:100

Other Notes

Small volumes of anti-RFFL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFFL purchase

MBS2528037 is an antibody targeting RFFL. Also known as RFFL Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RFFL. It may not necessarily be applicable to this product.

NCBI GI #

40226017

NCBI GeneID

117584

NCBI Accession #

AAH15681.2

UniProt Secondary Accession #

Q8NHW0; Q8TBY7; Q96BE6; E1P633

UniProt Related Accession #

Q8WZ73

NCBI Official Full Name

RFFL protein, partial

NCBI Official Synonym Full Names

ring finger and FYVE-like domain containing E3 ubiquitin protein ligase

NCBI Official Symbol

RFFL

NCBI Official Synonym Symbols

CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN

UniProt Protein Name

E3 ubiquitin-protein ligase rififylin

UniProt Synonym Protein Names

Caspase regulator CARP2; Caspases-8 and -10-associated RING finger protein 2; CARP-2; FYVE-RING finger protein Sakura; Fring; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34-like

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

RFFL

UniProt Synonym Gene Names

CARP-2; Fring

UniProt Entry Name

RFFL_HUMAN

NCBI Protein Information

E3 ubiquitin-protein ligase rififylin; FYVE-RING finger protein SAKURA; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; caspase regulator CARP2; caspases-8 and -10-associated RING finger protein 2; ring finger and FYVE-like domain containing 1

UniProt Comments for RFFL

RFFL: Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Vesicle

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: Golgi membrane; membrane; recycling endosome membrane; lysosome; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; endosome membrane; cytosol

Molecular Function: protein binding; protease binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; protein kinase binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; intracellular protein transport; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of TOR signaling pathway

Research Articles on RFFL

1. EGF, which signals through CRAF, and an activated BRAF mutant also activate PKC and stimulate cell migration through up-regulating RFFL expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RFFL antibody

Products by Pathway	Pathway Diagram
TNF Receptor Signaling Pathway antibodies	TNF Receptor Signaling Pathway Diagram

Diseases associated with anti-RFFL antibody

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>2 publications with RFFL and Disease Models, Animal
Hypertension Antibodies	>2 publications with RFFL and Hypertension
Proteinuria Antibodies	>1 publications with RFFL and Proteinuria
Inflammation Antibodies	>1 publications with RFFL and Inflammation
Fibrosis Antibodies	>1 publications with RFFL and Fibrosis
Necrosis Antibodies	>1 publications with RFFL and Necrosis

Organs/Tissues associated with anti-RFFL antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>2 publications with RFFL and Heart
Muscle Antibodies	>2 publications with RFFL and Muscle
Blood Antibodies	>1 publications with RFFL and Blood
Kidney Antibodies	>1 publications with RFFL and Kidney
Lung Antibodies	>1 publications with RFFL and Lung