Phosphate tris(acetoxymethyl)ester (PO4-AM3)

Typical analysis is better than 95% (MS). The product is not sterile and has not been tested for endotoxins

Micromolar quantities are determined by weight.0.33 ?mol of PO4-AM3 is stoichiometrically equivalentto 1 ?mol of a cyclic nucleotide-AM este.

PO4-AM3is sufficiently stable to be shipped at ambient temperature, however, it should be stored in the freezer (-20°Cnecessary, -80°C recommended).Please note that aqueous solutions are rather labile and should be freshly prepared immediately before use. Stock solutions in anhydrous DMSO or DMF shouldbe relatively stable when stored frozen at -20°C to -80°C.

314.2

PO4-AM3is a membrane- permeable prodrug of inorganic phosphate. The acetoxymethyl group s mask the charge d polar phosphate and thus render the molecule highly membrane - permeant. Inside the cell esterases release the highly polar inorganic phosphate (PO 4 3 - ) . In addition, the AM ester groups liberate acetic aci d and formaldehyde, two metabolites with potential biological functions. PO 4 - AM 3 is recommended as control reagent in nucleotide - AM ester applications to test for side effects of acetic acid and formaldehyde.

Due to its rather high lipophilicity, the solubility of PO4-AM3in water or buffers is limited. We suggest to use a small amount of anhydrous organic solvent such as anhydrous DMSO or DMF for preparation of 1-100 mM stock solutions, and to dilute withwater or buffer down to the concentrations required. In some cases, especially at high concentrations (~ 1mM), Pluronic?F-127 (Molecular Probes) can be useful to facilitate solubilization in water or physiological media. Please be sure to check for DMSO/DMF tolerance in your system. Since PO4-AM3is bioactivated by esterases, application to cell cultures should be performed without serum supplements (even heat-inactivated serum still contains active esterases!) in the media for at least 15 minutes. Otherwise, serum esterases may strongly reduce the cell-loading efficacy. Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.

C₉H₁₅O₁₀P

[148334-61-6]