8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel (Rp-8-AHA-cAMPS-Agarose)

The PKA inhibitor Rp-cAMPS and the corresponding agonistic Sp-cAMPS are immobilized to agarose via a hexyl spacer at position 8 of the adenine nucleobase.

Properties of Rp-8-AHA-cAMPS Agarose: The ligand of this gel represents an immmobilized form of the cyclic AMP antagonist Rp-cAMPS. Free Rp-cAMPS was found to prevent dissociation of protein kinase A I & II catalytic from regulatory subunits and thus to inhibit the cAMP signal pathway. In addition Rp-cAMPS is not hydrolyzed by mammalian phosphodiesterases. Both properties make this analogue to be an interesting ligand in affinity chromatography, especially when trying to purify protein kinase A holoenzyme, cyclic nucleotide- dependent phosphodiesterases or other cAMP binding proteins. Immobilized Rp-cAMPS can also be used as a stable general ligand with reduced affinity for various nucleotide binding proteins since POE degradation, the first metabolic step necessary in cyclic nucleotide metabolism, is blocked. Immobilized Rp-cAMPS is also available with a short aminoethylamino spacer; for other spacer types, attachment positions or gels please inquire.
If the target protein is absorbed too strongly on common cAMP agaroses, both gels can used as alternative due to the reduced binding affinity of phosphorothioate-modified cyclic nucleotides to most receptors. Also, the column can be useful for purification of cGMP receptors in case the protein sticks too tightly to a corresponding gel with immobilized cGMP.

Small volumes of 8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel (Rp-8-AHA-cAMPS-Agarose) biochemical vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS256231 is a biochemical targeting 8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel (Rp-8-AHA-cAMPS-Agarose).