3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP/ 3'-O-Ac-dTTP), sodium salt Biochemical – Cat# MBS256643

3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP/ 3'-O-Ac-dTTP), sodium salt (3'- O- Acetylthymidine- 5'- O- Triphosphate (3'-O-Ac-TTP / 3'-O-Ac-Dttp))

Name: 3'- O- Acetylthymidine- 5'- O- Triphosphate (3'-O-Ac-TTP / 3'-O-Ac-Dttp); MyBioSource
Brand: MyBioSource
SKU: MBS256643
Price: 585 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS256643

Ribose-modified TTP analogue. 3'-O-Ac-TTP is an analogue of the parent nucleotide 2'-deoxycytidine-5'-O-triphosphate (TTP / dTTP) in which the3'-hydroxy group is esterified by acetic acid. More details

$585 / 5 umol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP), Biochemical

Full Product Name

3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP/ 3'-O-Ac-dTTP), sodium salt

Product Synonym Names

3'-O-Ac-TTP; 3'-O-Ac-dTTP

Form/Format

Tetrasodium. Aqueous solution of the sodium salt (10 mM).

Purity/Purification

Typical purity is >95% (HPLC/ UV/ 267 nm) at time of quality control and packing. However, actual purity depends on storage and transport conditions. The product is not sterile and has not been tested for endotoxins.

Preparation and Storage

Store at -70 degree C
It is most stable when stored as aqueous solution in the freezer (-20 degree Celsius necessary, -80 degree recommended), however, at ambient temperature the compound slowly starts to decompose. Thus, in order to maintain its original high quality it is recommended to allow thawing only before using the product. If you will not use up the vial with one application, please aliquot the contents of the vial in order to avoid repeated freeze/thaw cycles for the rest. When making such aliquots, be sure to operate quickly and to freeze the vial again as soon as possible.
Shipping: Dry Ice

Molecular Weight

524.2

SMILES

[O-]P(OC[C@H]1O[C@@H](N2C=C(C)C(NC2=O)=O)C[C@@H]1OC(C)=O)(OP(OP([O-])([O-])=O)([O-])=O)=O.[Na+].[Na+].[Na+].[Na+]

CAS Number

90290-82-7

Formula

C??H??N?O??P?

Properties

Analogue of TTP with blocked 3'-hydroxy function, which can be used as a transient, pH-sensitive chain terminator during DNA polymerization. The acetyl group of 3'-O-Ac-TTP is sensitive towards alkaline conditions and can be split off at pH >12.5 within 30min. to allow further subsequent polymerization steps.

lambda max 267 nm, epsilon 9600, pH 7

Notes

Micromolar quantities are determined by UV at lambda max. When opening the tube please make sure that no liquid is lost within the cap. A short spin down in a bench centrifuge is recommended before use.
Since nucleoside triphosphates have multiple tasks in every organism, it is very likely that TTP analogues willinterfere with many cell regulation processes in vivo. However, due to the rather small quantities to work with, no health hazards have been reported.

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product References and Citations for 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP) biochemical

Koukhareva, I.; Lebedev, A., Anal. Chem., 81, 4955 - 4962 (2009): "3'-Protected 2'-Deoxynucleoside 5'-Triphosphates as a Tool forHeat-Triggered Activation of Polymerase Chain Reaction"
Marián, M., Microchem. J., 29, 219 - 227 (1984): "Acetyl derivatives of nucleoside 5?-triphosphates, I"
Hutter, D.; Kim, M.-J.; Karalkar, N.; Leal, N.A.; Chen, F.; Guggenheim, E.; Visalakshi, V.; Olejnik, J., Benner, S.A., Nucleosides,Nucleotides Nucleic Acids, 29, 879 - 895 (2010): "Labeled Nucleoside Triphosphates with Reversibly Terminating AminoalkoxylGroups"
Guo, J.; Xu, N.; Li, Z.; Zhang, S.; Wu, J.; Kim, D.H.; Marma, M.S.; Meng, Q.; Cao, H.; Li, X.; Shi, S.; Yu, L.; Kalachikov, S.;Russo, J.J.; Turro, N.J., Ju, J., Proc. Nat. Acad. Sci. USA, 105, 9135 - 9150 (2008): "Four-color DNA sequencing with 3'-Omodified nucleotide reversible terminators and chemically cleavable fluorescent dideoxynucleotides"
.Metzker, M.L.; Raghavachari, R.; Richards, S.; Jacutin, S.E.; Civitello, A.; Burgess, K.; Gibbs, R.A., Nucl. Acid Res., 22, 4259 -4267 (1994): "Termination of DNA synthesis by novel 3'-modified-deoxyribonucleoside 5'-triphosphates.

Other Notes

Small volumes of 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP) biochemical vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP) purchase

MBS256643 is a biochemical targeting 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP). Also known as 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP/ 3'-O-Ac-dTTP), sodium salt.

3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP/ 3'-O-Ac-dTTP), sodium salt (3'- O- Acetylthymidine- 5'- O- Triphosphate (3'-O-Ac-TTP / 3'-O-Ac-Dttp))

Product Overview

Structure of 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP) biochemical

Related Product Information for 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP) biochemical

Product References and Citations for 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP) biochemical

Other Notes

Searchable Terms for 3'- O- Acetylthymidine- 5'- O- triphosphate (3'-O-Ac-TTP / 3'-O-Ac-dTTP) purchase