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CK-18 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2579084

Cytokeratin 18 (CK18) has a molecular weight of 45 KDa, which is a low molecular weight type A cytokeratin. It is a marker of simple epithelium, including glandular epithelium. The stratified squamous epithelium is generally negative. CK18 is mainly used in… More details

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Product Overview

Product Name
CK-18, Polyclonal Antibody
Full Product Name
CK-18 Polyclonal Antibody
Product Gene Name
anti-CK-18 antibody
Product Synonym Names
Cell proliferation inducing gene 46 protein; CK 18; CYK 18; Cytokeratin 18; K1C18_HUMAN; KA18; Keratin 18; Keratin D; Keratin-18; Krt18
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4
Purity/Purification
Antigen Affinity Purification
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C Valid for 12 months. Avoid freeze/thaw cycles.
Molecular Weight
48,058 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (53342655..53346685). Location: 12q13
OMIM
148070
3D Structure
ModBase 3D Structure for P05783
Immunogen
Recombinant Human Keratin, type I cytoskeletal 18 protein

Product Categories/Family for anti-CK-18 antibody

Polyclonal Antibodies; Primary Antibodies; Polyclonal Antibodies; Cancer; Signal Transduction; Tags and Cell Markers
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CK-18 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-CK-18 antibody
WB: 1:500-1:1000
IHC: 1:500-1:1000
IF: 1:100-1:200

Western Blot (WB) of anti-CK-18 antibody

Western Blot analysis of Hela, Jurkat, A431 and k562 cells using CK-18 Polyclonal Antibody at dilution of 1:500
Rabbit Anti-CK-18 Polyclonal Antibody – Cat# MBS2579084

Western Blot (WB) of anti-CK-18 antibody

Western Blot analysis of Rat liver and Mouse liver using CK-18 Polyclonal Antibody at dilution of 1:500
Rabbit Anti-CK-18 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2579084

Immunohistochemistry (IHC) of anti-CK-18 antibody

Immunohistochemistry of paraffin-embedded Human liver using CK-18 Polyclonal Antibody at dilution of 1:100
Rabbit Anti-CK-18 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2579084

Immunohistochemistry (IHC) of anti-CK-18 antibody

Immunohistochemistry of paraffin-embedded Human breast using CK-18 Polyclonal Antibody at dilution of 1:100
Rabbit Anti-CK-18 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2579084

Immunohistochemistry (IHC) of anti-CK-18 antibody

Immunohistochemistry of paraffin-embedded Rat kidney using CK-18 Polyclonal Antibody at dilution of 1:100
Rabbit Anti-CK-18 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2579084

Immunohistochemistry (IHC) of anti-CK-18 antibody

Immunohistochemistry of paraffin-embedded Human prostate using CK-18 Polyclonal Antibody at dilution of 1:100.
Rabbit Anti-CK-18 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2579084

Immunofluorescence (IF) of anti-CK-18 antibody

Immunofluorescence analysis of Hela cells using CK-18 Polyclonal Antibody at dilution of 1:100
Rabbit Anti-CK-18 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS2579084

Immunofluorescence (IF) of anti-CK-18 antibody

Immunofluorescence analysis of U-2OS cells using CK-18 Polyclonal Antibody at dilution of 1:100
Rabbit Anti-CK-18 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS2579084

Other Notes

Small volumes of anti-CK-18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CK-18 purchase

MBS2579084 is an antibody targeting CK-18. Also known as CK-18 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CK-18. It may not necessarily be applicable to this product.

NCBI GI #
4557888
NCBI GeneID
3875
NCBI Accession #
NP_000215.1
UniProt Primary Accession #
P05783
UniProt Secondary Accession #
Q53G38; Q5U0N8; Q9BW26
UniProt Related Accession #
P05783
GenBank Nucleotide #
NM_000224.2
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CYK18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Synonym Protein Names
Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein

NCBI Summary for CK-18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CK-18

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; microtubule organizing center; intermediate filament

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; tumor necrosis factor-mediated signaling pathway; viral reproduction; intermediate filament cytoskeleton organization and biogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; negative regulation of apoptosis

Disease: Cirrhosis, Familial
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Research Articles on CK-18

1. K8/18 expression can influence the phenotype of epithelial cancer cells at a transcriptional level and supports the hypothesis that keratins play an active role in cancer progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CK-18 antibody

 Reference Product  PubMed Publications
 KRT8 antibody  >39 publications with CK-18 and KRT8
 CASP3 antibody  >5 publications with CK-18 and CASP3
 KRT5 antibody  >3 publications with CK-18 and KRT5
 EGFR antibody  >3 publications with CK-18 and EGFR
 DNAJB6 antibody  >1 publications with CK-18 and DNAJB6
 CASP7 antibody  >1 publications with CK-18 and CASP7
 CASP6 antibody  >1 publications with CK-18 and CASP6

Pathways associated with anti-CK-18 antibody

 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-CK-18 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >41 publications with CK-18 and Neoplasms
 Liver Diseases Antibodies  >28 publications with CK-18 and Liver Diseases
 Carcinoma Antibodies  >23 publications with CK-18 and Carcinoma
 Adenocarcinoma Antibodies  >13 publications with CK-18 and Adenocarcinoma
 Breast Neoplasms Antibodies  >12 publications with CK-18 and Breast Neoplasms
 Fibrosis Antibodies  >12 publications with CK-18 and Fibrosis
 Fatty Liver Antibodies  >9 publications with CK-18 and Fatty Liver
 Liver Cirrhosis Antibodies  >8 publications with CK-18 and Liver Cirrhosis
 Neoplasm Metastasis Antibodies  >8 publications with CK-18 and Neoplasm Metastasis
 Liver Neoplasms Antibodies  >7 publications with CK-18 and Liver Neoplasms

Organs/Tissues associated with anti-CK-18 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >34 publications with CK-18 and Liver
 Blood Antibodies  >18 publications with CK-18 and Blood
 Eye Antibodies  >6 publications with CK-18 and Eye
 Muscle Antibodies  >6 publications with CK-18 and Muscle
 Kidney Antibodies  >6 publications with CK-18 and Kidney
 Prostate Antibodies  >5 publications with CK-18 and Prostate
 Vascular Antibodies  >4 publications with CK-18 and Vascular
 Lymph Antibodies  >4 publications with CK-18 and Lymph
 Lymph Node Antibodies  >4 publications with CK-18 and Lymph Node
 Bladder Antibodies  >4 publications with CK-18 and Bladder
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