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Calretinin (CR) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2579944

Calretinin(CR) is an intracellular calcium binding protein. During the cerebellum development, immunohistochemical tests discovered that Calretinin began to be weakly expressed in purkinje cells, blue cells and dentate nucleus neurons in the later stage… More details

$420 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Calretinin (CR), Monoclonal Antibody
Full Product Name
Calretinin (CR) Monoclonal Antibody
Product Gene Name
Clonality
Monoclonal
Clone Number
YN00166m
Host
Mouse
Species Reactivity
Human
Preparation and Storage
The concentrated antibody should be stored at -20°C protecting from light. It is recommended to sub-pack into several small tubes for the first use. Take one tube each time. Avoid freeze / thaw cycles. The RTU antibody should be stored at 2~8°C protecting from light (Do not freeze). Please restore at 2~8°C immediately after each time use. Please follow this manual to store the antibody to avoid badly affecting the titer and effective service life of the product.
OMIM
114051
3D Structure
ModBase 3D Structure for P22676
Localization
Cytoplasm
Incubation
Incubate at RT (18-30°C) for 60 min or incubate at 4°C for overnight.
Notice
  1. The product should be operated by experienced researchers.
  2. This product is for Immunohistochemistry use only.
  3. The product should be followed by detection kits such as Secondary antibody and DAB kits.
  4. Protective measures should be taken to avoid contact with skin and eyes
  5. The product contains sodium azide as an antiseptic. Sodium azide can react with lead or copper to form an explosive metal azide. A large amount of water is used to avoid the formation of metal azides.
  6. This product is biological resource, and its treatment should meet the relevant requirements.
Judgment of positive result
The judgment of the immunohistochemical staining results should be observed under the microscope and taken by experienced researchers. The results of immunohistochemistry should be judged on the basis of tissue positive control and reagent negative control. Tissue positive control: Tissue section with confirmed antigen, the result should be positive.
Reagent negative control: Use PBS buffer instead of antibody incubation, the result should be negative.
Positive result (+): It refers to tan or brown staining on specific cells without background staining.
Negative result (-): It refers to no tan or brown staining results in the expected positive tissue cells.
>> Refer to the following
Tissue positive control (+), Reagent negative control (-), Test tissue (+): Indicates the antigen can be detected in the tested tissue.
Tissue positive control (+), Reagent negative control (-), Test tissue (-): Indicates that there is no or less antigen in the tested tissue.
>> Don’t refer to the following
Tissue positive control (+), Reagent negative control (+), Test tissue (+)
Tissue positive control (+), Reagent negative control (+), Test tissue (-)
Tissue positive control (-), Reagent negative control (-), Test tissue (+)
Tissue positive control (-), Reagent negative control (+), Test tissue (+)
Tissue positive control (-), Reagent negative control (-), Test tissue (-)
Tissue positive control (-), Reagent negative control (-), Test tissue (+)
Interpretation of test results
  1. The results of immunohistochemical staining must be established on the basis that the positive control gets a positive result and the negative control gets a negative result. The result of the experiment slice should be positive control (+) or reagent negative (-), or the result should be neglected
  2. The methods and time of antigen repair, incubation time and temperature may affect the test results. Please follow the experiment method strictly
  3. When the tissues are prepared to paraffin sections, please detect the tissue slides within one week to avoid false negative antigens.
Limitations of detection methods
Immunohistochemistry is a multi-step experimental process. Improper treatment of each step will affect the final results. Any positive staining results must be evaluated by an experienced researcher and refer to the sample cytomorphology and histopathological background. The value of the test results should also be comprehensively analyzed and judged by the experienced researcher in combination with other test results.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CR antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes for anti-CR antibody
This product is used for immunohistochemical staining of formalin fixed paraffin embedded or frozen sections.The recommended dilution of concentrated product is 1:50-1:200,and RTU is ready to use.Repair the antigens in pH9.0 EDTA solution by microwave or high-pressure boiling before incubating the antibodies.This product is the primary antibody required for immunohistochemistry experiment,which needs to be followed with secondary antibody,DAB kit,hematoxylin staining solution and other reagents.This product is suitable for the immunohistochemistry kits produced by various manufacturers

Testing Data of anti-CR antibody

Formalin-fixed, paraffin-embedded (FFPE) Human Vermiform appendix tissue with Calretinin (CR) Monoclonal Antibody.
Mouse Anti-CR Monoclonal Antibody – Cat# MBS2579944

Other Notes

Small volumes of anti-CR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CR purchase

MBS2579944 is an antibody targeting CR. Also known as Calretinin (CR) Monoclonal Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
calretinin
UniProt Protein Name
Calretinin
UniProt Synonym Protein Names
29 kDa calbindin
Protein Family
UniProt Gene Name
CALB2
UniProt Synonym Gene Names
CAB29; CR
UniProt Entry Name
CALB2_HUMAN

UniProt Comments for CR

CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: cytoplasm; gap junction; nucleus

Molecular Function: calcium ion binding

Biological Process: cytosolic calcium ion homeostasis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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