• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Hoechst 33342 Blue Nuclear DNA dye Reagent – Supplied at 200 ug/mL.

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258061

Description: Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pyknotic nuclei in apoptotic cells and for cell cycle… More details

$165 / 1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hoechst 33342 Blue Nuclear DNA dye, Reagent
Full Product Name
Hoechst 33342 blue nuclear DNA dye
Product Synonym Names
Hoechst 33342 Fluorescent Nucleic Acid Stain
Form/Format
Supplied at 200 ug/mL.
Preparation and Storage
Store at 2-8 degree C.
pH
4.0 + 1.0
Excitation/Emission
350 nm / 480 nm
Applications Tested/Suitable for Hoechst 33342 Blue Nuclear DNA dye reagent
Flow Cytometer, Fluorescence Microscope
1. Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 uL Hoechst to 300 uL of cells.
2. Incubate 10-20 minutes at room temperature.
3. Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.
4. Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.
5. When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.

Other Notes

Small volumes of Hoechst 33342 Blue Nuclear DNA dye reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hoechst 33342 Blue Nuclear DNA dye purchase

MBS258061 is a reagent targeting Hoechst 33342 Blue Nuclear DNA dye.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000