STOPT Reagent – Supplied at 1X.

Description: Stop Solution for TMB Substrates (STOPT) is a ready-to-use liquid acidic stop solution suitable for use with our TMB substrates. TMB substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution for TMB Substrates, the chromogen changes from blue to yellow. This product is suitable for all endpoint ELISAs using a TMB substrate for color development.

Background: Stop Solution for TMB Substrates (STOPT) is a ready-to-use liquid acidic stop solution suitable for use with our TMB substrates. This product is suitable for all endpoint ELISAs using a TMB substrate for color development.

TMB substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution for TMB Substrates, the chromogen changes from blue to yellow, and hence the absorbance from 620 nm to 450 nm.

Small volumes of STOPT reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS258088 is a reagent targeting STOPT. Also known as Stop Solution for TMB Microwell Substrate.