CyGEL Sustain Pack Reagent – Cellular Imaging

Description: CyGEL Sustain is a novel thermoreversible gel that is compatible with extended imaging of live cells and zebrafish embryos for several hours depending on cell/organism type. Its special formulation is optimized for the addition of RPMI and similar culture media. It can be used to immobilize non-adherent cells and beads by simple warming and conversely allowing their recovery by simple cooling.

Background: CyGEL is a thermo-reversible hydrogel. It is a liquid when cooled and gel when warm. It is compatible with and non-toxic to cells, tissues and model organisms such as zebrafish. CyGEL Sustain Thermoreversible Gel has been formulated to transition from liquid to gel at room temperature, allowing immobilization of non-adherent (suspension) cells, beads, micro-tissues, parasites, worms, and embryos (such as drosophila), which can be recovered non-destructively by cooling and/or over-dilution using cold buffer.

CyGel Sustain has applications in microplate cytometry, high content screening, fluorescence microscopy, in vitro toxicology, and apoptosis studies.

CyGEL Sustain can be used in the analysis of cell death and apoptosis. Immobilizing cells with CyGEL Sustain is a convenient method for analyzing live and dead cell populations generated from adherent cultures or suspension cultures. FLICA caspase inhibitor reagents, DRAQ5 (cell-permeant DNA dye) and DRAQ7 (live cell impermeant DNA dye) fluorescent reagents can be incorporated into CyGEL to provide in situ staining of cells or used as pre-stains for cell samples prior to immobilization. An interesting feature of DRAQ5 and DRAQ7 dyes is the intense blue color imparted to cells successfully stained-these cells can be readily seen in the cell pellets of suspension cells prepared by centrifugation-providing a visual check on the efficiency of cell harvesting if this is used prior to preparation with CyGEL Sustain.

Small volumes of CyGEL Sustain Pack reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS258368 is a reagent targeting CyGEL Sustain Pack.