Estrogens ELISA Kit for Human

Principle of the Assay: The kit assay Human E level in the sample, use Purified Human E antibody to coat microtiter plate wells, make solid-phase antibody, then add E to wells, Combined E antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of Estrogens elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8579261 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estrogens, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Estrogens. The ELISA analytical biochemical technique of the MBS8579261 kit is based on Estrogens antibody-Estrogens antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Estrogens antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Estrogens. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).