Epidermal Keratin (EK) ELISA Kit for Human

Name: Human EK ELISA Kit; Epidermal Keratin; MyBioSource
Brand: MyBioSource
SKU: MBS263921
Price: 340 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS263921

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human EK monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details

$340 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

epidermal keratin (EK), ELISA Kit

Full Product Name

Human epidermal keratin (EK) ELISA Kit

Product Gene Name

EK elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Detection Range

10 ng/mL-0.156 ng/mL

Sensitivity

Up to 0.05 ng/mL

Specificity

No cross-reaction with other factors.

Preparation and Storage

Store all reagents at 2-8 degree C.

Inter-assay Precision

<= 12%

Intra-assay Precision

<= 8%

Molecular Weight

51,561 Da

OMIM

601001

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of EK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EK purchase

MBS263921 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the epidermal keratin (EK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EK. The ELISA analytical biochemical technique of the MBS263921 kit is based on EK antibody-EK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EK. It may not necessarily be applicable to this product.

NCBI GI #

177139

NCBI GeneID

3861

NCBI Accession #

AAA96689.1

UniProt Secondary Accession #

Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4

UniProt Related Accession #

P02533

NCBI Official Full Name

epidermal type I keratin, partial

NCBI Official Synonym Full Names

keratin 14

NCBI Official Symbol

KRT14

NCBI Official Synonym Symbols

K14; NFJ; CK14; EBS3; EBS4

UniProt Protein Name

Keratin, type I cytoskeletal 14

UniProt Synonym Protein Names

Cytokeratin-14; CK-14; Keratin-14; K14

UniProt Gene Name

KRT14

UniProt Synonym Gene Names

CK-14; K14

UniProt Entry Name

K1C14_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 14; CK-14; keratin-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)

NCBI Summary for EK

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

UniProt Comments for EK

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: keratin filament; cytoplasm; intermediate filament; cytosol; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development; epithelial cell differentiation; response to zinc ion; intermediate filament bundle assembly; response to ionizing radiation; hair cycle; aging

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type

Research Articles on EK

1. BerEp4 alone is unreliable for differentiation between BCCm (basal cell carcinoma with squamous metaplasia) and bSCC (basaloid squamous cell carcinoma). The addition of either CK14 or CK17 will augment BCCm versus bSCC differential diagnosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EK elisa kit

Reference Product	PubMed Publications
KRT5 elisa kit	>80 publications with EK and KRT5
EGFR elisa kit	>7 publications with EK and EGFR
DST elisa kit	>3 publications with EK and DST
IVL elisa kit	>1 publications with EK and IVL
CREBBP elisa kit	>1 publications with EK and CREBBP

Pathways associated with EK elisa kit

Products by Pathway	Pathway Diagram
Corticotropin-releasing Hormone Pathway antibodies	Corticotropin-releasing Hormone Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with EK elisa kit

Disease Name	Pubmed Publications
Epidermolysis Bullosa Simplex Antibodies	>93 publications with EK and Epidermolysis Bullosa Simplex
Carcinoma Antibodies	>58 publications with EK and Carcinoma
Carcinoma, Squamous Cell Antibodies	>38 publications with EK and Carcinoma, Squamous Cell
Disease Models, Animal Antibodies	>23 publications with EK and Disease Models, Animal
Hyperplasia Antibodies	>19 publications with EK and Hyperplasia
Breast Neoplasms Antibodies	>17 publications with EK and Breast Neoplasms
Necrosis Antibodies	>13 publications with EK and Necrosis
Cell Transformation, Neoplastic Antibodies	>13 publications with EK and Cell Transformation, Neoplastic
Inflammation Antibodies	>12 publications with EK and Inflammation
Neoplasms, Experimental Antibodies	>11 publications with EK and Neoplasms, Experimental

Organs/Tissues associated with EK elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>151 publications with EK and Skin
Mouth Antibodies	>15 publications with EK and Mouth
Lung Antibodies	>9 publications with EK and Lung
Esophagus Antibodies	>8 publications with EK and Esophagus
Eye Antibodies	>7 publications with EK and Eye
Mammary Gland Antibodies	>7 publications with EK and Mammary Gland
Connective Tissue Antibodies	>6 publications with EK and Connective Tissue
Liver Antibodies	>6 publications with EK and Liver
Cervix Antibodies	>5 publications with EK and Cervix
Bladder Antibodies	>4 publications with EK and Bladder