Human SRF / IF ELISA Kit – Cat# MBS264052

Skin Reactive Factor / Inflammatory Factor (SRF / if) (SRF / IF) ELISA Kit for Human

Name: Human SRF / IF ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS264052
Price: 340 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS264052

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SRF/IF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and… More details

$340 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

skin reactive factor / inflammatory factor (SRF / IF), ELISA Kit

Full Product Name

Human skin reactive factor / inflammatory factor (SRF / IF) ELISA Kit

Product Gene Name

SRF / IF elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma or cell culture supernatant

Detection Range

1000 pg/ml-15.6 pg/ml

Sensitivity

Up to 5 pg/ml

Specificity

No cross-reaction with other factors.

Preparation and Storage

Store all reagents at 2-8 degree C.

Inter-assay Precision

<= 12%

Intra-assay Precision

<= 8%

Molecular Weight

124,076 Da

OMIM

605010

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SRF / IF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SRF / IF purchase

MBS264052 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the skin reactive factor / inflammatory factor (SRF / IF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SRF / IF. The ELISA analytical biochemical technique of the MBS264052 kit is based on SRF / IF antibody-SRF / IF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SRF / IF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SRF / IF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SRF / IF. It may not necessarily be applicable to this product.

NCBI GI #

189163504

NCBI GeneID

11005

NCBI Accession #

NP_001121171.1

UniProt Secondary Accession #

O75770; Q3LX95; Q3LX96; Q3LX97; Q96PP2; Q96PP3; A8MYE8; B7WPB7; D6REN5

UniProt Related Accession #

Q9NQ38

GenBank Nucleotide #

NM_001127699.1

NCBI Official Full Name

serine protease inhibitor Kazal-type 5 isoform c

NCBI Official Synonym Full Names

serine peptidase inhibitor, Kazal type 5

NCBI Official Symbol

SPINK5

NCBI Official Synonym Symbols

NS; NETS; LEKTI; LETKI; VAKTI

UniProt Protein Name

Serine protease inhibitor Kazal-type 5

UniProt Synonym Protein Names

Lympho-epithelial Kazal-type-related inhibitor; LEKTI

UniProt Gene Name

SPINK5

UniProt Synonym Gene Names

LEKTI

UniProt Entry Name

ISK5_HUMAN

NCBI Protein Information

serine protease inhibitor Kazal-type 5; serine protease inhibitor, Kazal type 5; lymphoepithelial Kazal-type-related inhibitor; lympho-epithelial Kazal-type-related inhibitor

NCBI Summary for SRF / IF

This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for SRF / IF

SPINK5: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. Defects in SPINK5 are the cause of Netherton syndrome (NETH). NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: endoplasmic reticulum membrane; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; extracellular region; cell cortex; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; epithelial cell differentiation; negative regulation of immune response; epidermal cell differentiation; hair cell differentiation; regulation of T cell differentiation; anagen

Disease: Ige Responsiveness, Atopic; Netherton Syndrome

Research Articles on SRF / IF

1. Herein we report three patients with Netherton syndrome who had growth retardation associated with GH deficiency and responded well to GH therapy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SRF / IF elisa kit

Reference Product	PubMed Publications
KLK7 elisa kit	>23 publications with SRF / IF and KLK7
KLK5 elisa kit	>21 publications with SRF / IF and KLK5
DSG1 elisa kit	>10 publications with SRF / IF and DSG1
FLG elisa kit	>7 publications with SRF / IF and FLG
ST14 elisa kit	>1 publications with SRF / IF and ST14

Diseases associated with SRF / IF elisa kit

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>120 publications with SRF / IF and Skin Diseases
Netherton Syndrome Antibodies	>95 publications with SRF / IF and Netherton Syndrome
Congenital Abnormalities Antibodies	>69 publications with SRF / IF and Congenital Abnormalities
Inflammation Antibodies	>14 publications with SRF / IF and Inflammation
Disease Models, Animal Antibodies	>3 publications with SRF / IF and Disease Models, Animal
Necrosis Antibodies	>3 publications with SRF / IF and Necrosis
Prenatal Injuries Antibodies	>1 publications with SRF / IF and Prenatal Injuries
Prenatal Exposure Delayed Effects Antibodies	>1 publications with SRF / IF and Prenatal Exposure Delayed Effects
Hyperplasia Antibodies	>1 publications with SRF / IF and Hyperplasia
Drug-Related Side Effects and Adverse Reactions Antibodies	>1 publications with SRF / IF and Drug-Related Side Effects and Adverse Reactions

Organs/Tissues associated with SRF / IF elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>105 publications with SRF / IF and Skin
Kidney Antibodies	>1 publications with SRF / IF and Kidney
Ovary Antibodies	>1 publications with SRF / IF and Ovary
Lung Antibodies	>1 publications with SRF / IF and Lung
Trachea Antibodies	>1 publications with SRF / IF and Trachea
Eye Antibodies	>1 publications with SRF / IF and Eye
Mouth Antibodies	>1 publications with SRF / IF and Mouth