Nitric Oxide Synthase (NOS) ELISA Kit for Porcine

Name: Porcine NOS ELISA Kit; Nitric Oxide Synthase; MyBioSource
Brand: MyBioSource
SKU: MBS264385
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS264385

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine NOS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and… More details

$370 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

nitric oxide synthase (NOS), ELISA Kit

Full Product Name

Porcine nitric oxide synthase (NOS) ELISA KIT

Product Gene Name

NOS elisa kit

Species Reactivity

Porcine

Assay Type

Sandwich

Samples

Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NOS concentration

Detection Range

100 U/ml-1.56 U/ml

Sensitivity

0.5U/ml.

Specificity

No cross-reaction with other factors.

Preparation and Storage

Store all reagents at 2-8 degree C.

Inter-assay Precision

<= 12%

Intra-assay Precision

<= 8%

Molecular Weight

67,876 Da

OMIM

163729

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOS purchase

MBS264385 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nitric oxide synthase (NOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NOS. The ELISA analytical biochemical technique of the MBS264385 kit is based on NOS antibody-NOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NOS. It may not necessarily be applicable to this product.

NCBI GI #

4262036

NCBI GeneID

4846

NCBI Accession #

AAD14336.1

UniProt Secondary Accession #

Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2

UniProt Related Accession #

P29474

NCBI Official Full Name

nitric oxide synthase, partial

NCBI Official Synonym Full Names

nitric oxide synthase 3 (endothelial cell)

NCBI Official Symbol

NOS3

NCBI Official Synonym Symbols

eNOS; ECNOS

UniProt Protein Name

Nitric oxide synthase, endothelial

UniProt Synonym Protein Names

Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

Protein Family

D-nopaline dehydrogenase

UniProt Gene Name

NOS3

UniProt Synonym Gene Names

cNOS; eNOS; NOSIII

UniProt Entry Name

NOS3_HUMAN

NCBI Protein Information

nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21

NCBI Summary for NOS

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

UniProt Comments for NOS

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: calmodulin binding; actin monomer binding; protein binding; FAD binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; FMN binding; iron ion binding; heme binding; NADP binding; cadmium ion binding

Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of blood pressure; regulation of the force of heart contraction by chemical signal; negative regulation of hydrolase activity; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; nitric oxide biosynthetic process; regulation of blood vessel size; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia

Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic

Research Articles on NOS

1. study to determine the association between the eNOS 4a/b polymorphism and breast cancer in Mexican women; genotypes a/a-a/b are associated with breast cancer susceptibility in the analyzed samples from the Mexican population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NOS elisa kit

Products by Pathway	Pathway Diagram
ACE Inhibitor Pathway antibodies	ACE Inhibitor Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Angiogenesis Pathway antibodies	Angiogenesis Pathway Diagram
Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Endothelin Pathway antibodies	Endothelin Pathway Diagram

Diseases associated with NOS elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>1908 publications with NOS and Cardiovascular Diseases
Hypertension Antibodies	>943 publications with NOS and Hypertension
Myocardial Ischemia Antibodies	>514 publications with NOS and Myocardial Ischemia
Ischemia Antibodies	>492 publications with NOS and Ischemia
Atherosclerosis Antibodies	>423 publications with NOS and Atherosclerosis
Coronary Artery Disease Antibodies	>346 publications with NOS and Coronary Artery Disease
Arteriosclerosis Antibodies	>321 publications with NOS and Arteriosclerosis
Reperfusion Injury Antibodies	>247 publications with NOS and Reperfusion Injury
Myocardial Infarction Antibodies	>222 publications with NOS and Myocardial Infarction
Neoplasms Antibodies	>198 publications with NOS and Neoplasms

Organs/Tissues associated with NOS elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>3282 publications with NOS and Vascular
Blood Antibodies	>2461 publications with NOS and Blood
Heart Antibodies	>1134 publications with NOS and Heart
Kidney Antibodies	>479 publications with NOS and Kidney
Brain Antibodies	>405 publications with NOS and Brain
Lung Antibodies	>347 publications with NOS and Lung
Liver Antibodies	>242 publications with NOS and Liver
Embryonic Tissue Antibodies	>199 publications with NOS and Embryonic Tissue
Bone Antibodies	>129 publications with NOS and Bone
Skin Antibodies	>90 publications with NOS and Skin