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15-Lipoxygenase (15-Lo / LOX) (15-LO / LOX) ELISA Kit for Chicken

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS265000

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken 15-LO/LOX monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples… More details

$370 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
15-lipoxygenase (15-LO / LOX), ELISA Kit
Full Product Name
Chicken 15-lipoxygenase (15-LO / LOX) ELISA Kit
Product Gene Name
15-LO / LOX elisa kit
Species Reactivity
Chicken
Assay Type
Quantitative Sandwich
Samples
Serum, plasma or cell culture supernatant
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
Up to 0.06 ng/ml.
Specificity
No cross-reaction with other factors.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
74,804 Da
OMIM
152392
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Standard Curve/Testing Data of 15-LO / LOX elisa kit

Chicken 15-LO / LOX ELISA Kit – Cat# MBS265000

Other Notes

Small volumes of 15-LO / LOX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 15-LO / LOX purchase

MBS265000 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 15-lipoxygenase (15-LO / LOX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 15-LO / LOX. The ELISA analytical biochemical technique of the MBS265000 kit is based on 15-LO / LOX antibody-15-LO / LOX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 15-LO / LOX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 15-LO / LOX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for 15-LO / LOX. It may not necessarily be applicable to this product.

NCBI GI #
307135
NCBI GeneID
246
NCBI Accession #
AAA36182.1
UniProt Secondary Accession #
Q8N6R7; Q99657; A8K2P4
UniProt Related Accession #
P16050
NCBI Official Full Name
15-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase
NCBI Official Symbol
ALOX15
NCBI Official Synonym Symbols
12-LOX; 15LOX-1; 15-LOX-1
UniProt Protein Name
Arachidonate 15-lipoxygenase
UniProt Synonym Protein Names
12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (EC:1.13.11.31); 12-LOX; Arachidonate omega-6 lipoxygenase
UniProt Gene Name
ALOX15
UniProt Synonym Gene Names
LOG15; 15-LOX; 15-LOX-1; 12-LOX
UniProt Entry Name
LOX15_HUMAN
NCBI Protein Information
arachidonate 15-lipoxygenase; 15-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type

UniProt Comments for 15-LO / LOX

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: EC 1.13.11.33; Oxidoreductase; Motility/polarity/chemotaxis; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; EC 1.13.11.31

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: protein binding; arachidonate 12-lipoxygenase activity; phosphatidylinositol-4,5-bisphosphate binding; hepoxilin A3 synthase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; bone mineralization; apoptotic cell clearance
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Research Articles on 15-LO / LOX

1. Positive ALOX15 expression is more prevalent in eosinophilic esophagitis than in gastroesophageal reflux disease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with 15-LO / LOX elisa kit

 Reference Product  PubMed Publications
 ALOX5 elisa kit  >199 publications with 15-LO / LOX and ALOX5
 PTGS2 elisa kit  >47 publications with 15-LO / LOX and PTGS2
 ALOX15B elisa kit  >42 publications with 15-LO / LOX and ALOX15B
 PTGS1 elisa kit  >25 publications with 15-LO / LOX and PTGS1
 PLA2G4A elisa kit  >15 publications with 15-LO / LOX and PLA2G4A
 STAT6 elisa kit  >12 publications with 15-LO / LOX and STAT6
 ALOX12B elisa kit  >3 publications with 15-LO / LOX and ALOX12B

Pathways associated with 15-LO / LOX elisa kit

 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Serotonergic Synapse Pathway antibodies  Serotonergic Synapse Pathway Diagram

Diseases associated with 15-LO / LOX elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >188 publications with 15-LO / LOX and Inflammation
 Neoplasms Antibodies  >147 publications with 15-LO / LOX and Neoplasms
 Atherosclerosis Antibodies  >131 publications with 15-LO / LOX and Atherosclerosis
 Hypertension Antibodies  >31 publications with 15-LO / LOX and Hypertension
 Necrosis Antibodies  >26 publications with 15-LO / LOX and Necrosis
 Kidney Diseases Antibodies  >22 publications with 15-LO / LOX and Kidney Diseases
 Heart Diseases Antibodies  >17 publications with 15-LO / LOX and Heart Diseases
 Drug Toxicity Antibodies  >16 publications with 15-LO / LOX and Drug Toxicity
 Fibrosis Antibodies  >13 publications with 15-LO / LOX and Fibrosis
 Arthritis, Rheumatoid Antibodies  >12 publications with 15-LO / LOX and Arthritis, Rheumatoid

Organs/Tissues associated with 15-LO / LOX elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >98 publications with 15-LO / LOX and Lung
 Prostate Antibodies  >45 publications with 15-LO / LOX and Prostate
 Trachea Antibodies  >17 publications with 15-LO / LOX and Trachea
 Intestine Antibodies  >15 publications with 15-LO / LOX and Intestine
 Embryonic Tissue Antibodies  >10 publications with 15-LO / LOX and Embryonic Tissue
 Adipose Tissue Antibodies  >9 publications with 15-LO / LOX and Adipose Tissue
 Placenta Antibodies  >8 publications with 15-LO / LOX and Placenta
 Lymph Node Antibodies  >6 publications with 15-LO / LOX and Lymph Node
 Testis Antibodies  >3 publications with 15-LO / LOX and Testis
 Mouth Antibodies  >2 publications with 15-LO / LOX and Mouth
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