Phospholipase A2 (PLA2) ELISA Kit for Human

Name: Human PLA2 ELISA Kit; Phospholipase A2; MyBioSource
Brand: MyBioSource
SKU: MBS265255
Price: 340 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS265255

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human PLA2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details

$340 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phospholipase A2 (PLA2), ELISA Kit

Full Product Name

Human Phospholipase A2 (PLA2) ELISA Kit

Product Gene Name

PLA2 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Detection Range

100 ng/mL-1.56 ng/mL

Sensitivity

Up to 0.5 ng/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Inter-assay Precision

<= 12%

Intra-assay Precision

<= 8%

Molecular Weight

16,360 Da

OMIM

172410

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLA2 purchase

MBS265255 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipase A2 (PLA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLA2. The ELISA analytical biochemical technique of the MBS265255 kit is based on PLA2 antibody-PLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PLA2. It may not necessarily be applicable to this product.

NCBI GI #

4505847

NCBI GeneID

5319

NCBI Accession #

NP_000919.1

UniProt Secondary Accession #

Q3KPI1; B2R4H5

UniProt Related Accession #

P04054

GenBank Nucleotide #

NM_000928.2

NCBI Official Full Name

phospholipase A2

NCBI Official Synonym Full Names

phospholipase A2, group IB (pancreas)

NCBI Official Symbol

PLA2G1B

NCBI Official Synonym Symbols

PLA2; PLA2A; PPLA2

UniProt Protein Name

Phospholipase A2

UniProt Synonym Protein Names

Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B

Protein Family

Protein terminal ear1

UniProt Gene Name

PLA2G1B

UniProt Synonym Gene Names

PLA2; PLA2A; PPLA2

UniProt Entry Name

PA21B_HUMAN

NCBI Protein Information

phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B

NCBI Summary for PLA2

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

UniProt Comments for PLA2

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - glycerophospholipid; Phospholipase; Secreted; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; DNA replication; Secreted, signal peptide; Lipid Metabolism - ether lipid

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space; cell surface; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; bile acid binding; calcium ion binding; receptor binding

Biological Process: positive regulation of immune response; activation of MAPK activity; activation of phospholipase A2; neutrophil mediated immunity; glucose transport; signal transduction; activation of NF-kappaB transcription factor; phosphatidylcholine metabolic process; positive regulation of fibroblast proliferation; phospholipid metabolic process; fatty acid biosynthetic process; multicellular organismal lipid catabolic process; neutrophil chemotaxis; glycerophospholipid biosynthetic process; actin filament organization; arachidonic acid secretion; leukotriene biosynthetic process; defense response to Gram-positive bacterium; positive regulation of protein secretion; cellular response to insulin stimulus; innate immune response in mucosa; antibacterial humoral response; phosphatidic acid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA replication; interleukin-8 production

Research Articles on PLA2

1. TNF-alpha-induced cPLA2 expression and PGE2 release were mediated through a Jak2/PDGFR/PI3K/Akt/p42/p44 MAPK/Elk-1 pathway in human lung epithelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PLA2 elisa kit

Reference Product	PubMed Publications
PTGS2 elisa kit	>2 publications with PLA2 and PTGS2
MAPK1 elisa kit	>1 publications with PLA2 and MAPK1

Pathways associated with PLA2 elisa kit

Products by Pathway	Pathway Diagram
Acyl Chain Remodelling Of PC Pathway antibodies	Acyl Chain Remodelling Of PC Pathway Diagram
Acyl Chain Remodelling Of PE Pathway antibodies	Acyl Chain Remodelling Of PE Pathway Diagram
Acyl Chain Remodelling Of PG Pathway antibodies	Acyl Chain Remodelling Of PG Pathway Diagram
Acyl Chain Remodelling Of PI Pathway antibodies	Acyl Chain Remodelling Of PI Pathway Diagram
Acyl Chain Remodelling Of PS Pathway antibodies	Acyl Chain Remodelling Of PS Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Ether Lipid Metabolism Pathway antibodies	Ether Lipid Metabolism Pathway Diagram
Ether Lipid Metabolism Pathway antibodies	Ether Lipid Metabolism Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram

Diseases associated with PLA2 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>3 publications with PLA2 and Inflammation
Fatty Liver Antibodies	>2 publications with PLA2 and Fatty Liver
Carcinoma, Hepatocellular Antibodies	>2 publications with PLA2 and Carcinoma, Hepatocellular
Hypertension Antibodies	>2 publications with PLA2 and Hypertension
Neoplasms Antibodies	>2 publications with PLA2 and Neoplasms
Seizures Antibodies	>2 publications with PLA2 and Seizures
Liver Diseases Antibodies	>2 publications with PLA2 and Liver Diseases
Hemorrhage Antibodies	>1 publications with PLA2 and Hemorrhage
Drug Toxicity Antibodies	>1 publications with PLA2 and Drug Toxicity
Adenocarcinoma Antibodies	>1 publications with PLA2 and Adenocarcinoma

Organs/Tissues associated with PLA2 elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>5 publications with PLA2 and Pancreas
Testis Antibodies	>2 publications with PLA2 and Testis
Kidney Antibodies	>1 publications with PLA2 and Kidney