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Alpha-Enolase (alphaENOL) ELISA Kit for Porcine

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS266509

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine alphaENOL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples… More details

$370 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
alpha-enolase (alphaENOL), ELISA Kit
Full Product Name
Porcine alpha-enolase (alphaENOL) ELISA Kit
Product Gene Name
alphaENOL elisa kit
Product Synonym Names
Porcine a-enolase (aENOL) ELISA Kit
Species Reactivity
Porcine
Assay Type
Sandwich
Samples
Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant alphaENOL concentration
Detection Range
10 ng/ml-0.156 ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Molecular Weight
47,128 Da
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of alphaENOL elisa kit

Porcine alphaENOL ELISA Kit – Cat# MBS266509

Other Notes

Small volumes of alphaENOL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for alphaENOL purchase

MBS266509 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-enolase (alphaENOL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alphaENOL. The ELISA analytical biochemical technique of the MBS266509 kit is based on alphaENOL antibody-alphaENOL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alphaENOL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alphaENOL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for alphaENOL. It may not necessarily be applicable to this product.

NCBI GI #
158186649
NCBI GeneID
24333
NCBI Accession #
NP_036686.2
UniProt Secondary Accession #
Q66HI3; Q6AYV3; Q6P504
UniProt Related Accession #
P04764
GenBank Nucleotide #
NM_012554.3
NCBI Official Full Name
alpha-enolase
NCBI Official Synonym Full Names
enolase 1, (alpha)
NCBI Official Symbol
Eno1
NCBI Official Synonym Symbols
Nne
UniProt Protein Name
Alpha-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
UniProt Gene Name
Eno1
UniProt Synonym Gene Names
Eno-1; NNE
UniProt Entry Name
ENOA_RAT
NCBI Protein Information
alpha-enolase; alpha enolase; non-neural enolase; enolase 1, alpha non-neuron; 2-phospho-D-glycerate hydro-lyase

UniProt Comments for alphaENOL

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transcription, coactivator/corepressor; Lyase; EC 4.2.1.11

Cellular Component: extrinsic to plasma membrane; extracellular space; neuron projection; phosphopyruvate hydratase complex; membrane; cytoplasm; nucleus

Molecular Function: protein homodimerization activity; RNA binding; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: glycolysis; in utero embryonic development; response to virus; negative regulation of cell growth; positive regulation of binding; negative regulation of transcription, DNA-dependent
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Research Articles on alphaENOL

1. this research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with alpha-enolase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with alphaENOL elisa kit

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram

Diseases associated with alphaENOL elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >69 publications with alphaENOL and Neoplasms
 Carcinoma Antibodies  >33 publications with alphaENOL and Carcinoma
 Nervous System Diseases Antibodies  >17 publications with alphaENOL and Nervous System Diseases
 Brain Diseases Antibodies  >15 publications with alphaENOL and Brain Diseases
 Adenocarcinoma Antibodies  >13 publications with alphaENOL and Adenocarcinoma
 Breast Neoplasms Antibodies  >12 publications with alphaENOL and Breast Neoplasms
 Arthritis, Rheumatoid Antibodies  >12 publications with alphaENOL and Arthritis, Rheumatoid
 Carcinoma, Non-Small-Cell Lung Antibodies  >11 publications with alphaENOL and Carcinoma, Non-Small-Cell Lung
 Inflammation Antibodies  >10 publications with alphaENOL and Inflammation
 Liver Neoplasms Antibodies  >7 publications with alphaENOL and Liver Neoplasms

Organs/Tissues associated with alphaENOL elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >28 publications with alphaENOL and Brain
 Lung Antibodies  >26 publications with alphaENOL and Lung
 Liver Antibodies  >14 publications with alphaENOL and Liver
 Muscle Antibodies  >13 publications with alphaENOL and Muscle
 Eye Antibodies  >12 publications with alphaENOL and Eye
 Heart Antibodies  >7 publications with alphaENOL and Heart
 Kidney Antibodies  >5 publications with alphaENOL and Kidney
 Ovary Antibodies  >4 publications with alphaENOL and Ovary
 Connective Tissue Antibodies  >3 publications with alphaENOL and Connective Tissue
 Prostate Antibodies  >3 publications with alphaENOL and Prostate
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