Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit for Porcine

Name: Porcine eNOS ELISA Kit; Endothelial Nitric Oxide Synthase; MyBioSource
Brand: MyBioSource
SKU: MBS266606
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS266606

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine eNOS monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details

$370 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Endothelial Nitric Oxide Synthase (eNOS), ELISA Kit

Full Product Name

Porcine Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit

Product Gene Name

eNOS elisa kit

Species Reactivity

Porcine

Assay Type

Quantitative Sandwich

Samples

Porcine serum, plasma or cell culture supernatant

Detection Range

10 ng/mL-0.156 ng/mL

Sensitivity

Up to 0.05 ng/mL

Preparation and Storage

Store all reagents at 2-8 degree C.

Inter-assay Precision

<= 12%

Intra-assay Precision

<= 8%

Molecular Weight

67,876 Da

OMIM

163729

3D Structure

ModBase 3D Structure for P29474

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of eNOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eNOS purchase

MBS266606 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing eNOS. The ELISA analytical biochemical technique of the MBS266606 kit is based on eNOS antibody-eNOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect eNOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, eNOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product.

NCBI GI #

825652

NCBI GeneID

4846

NCBI Accession #

CAA53950.1

UniProt Primary Accession #

P29474

UniProt Secondary Accession #

Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2

UniProt Related Accession #

P29474

NCBI Official Full Name

endothelial nitric oxide synthase

NCBI Official Synonym Full Names

nitric oxide synthase 3 (endothelial cell)

NCBI Official Symbol

NOS3

NCBI Official Synonym Symbols

eNOS; ECNOS

UniProt Protein Name

Nitric oxide synthase, endothelial

UniProt Synonym Protein Names

Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

UniProt Gene Name

NOS3

UniProt Synonym Gene Names

cNOS; eNOS; NOSIII

UniProt Entry Name

NOS3_HUMAN

NCBI Protein Information

nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21

NCBI Summary for eNOS

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

UniProt Comments for eNOS

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; nitric-oxide synthase activity; NADPH-hemoprotein reductase activity; FMN binding; iron ion binding; heme binding; NADP binding; cadmium ion binding

Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of blood pressure; negative regulation of hydrolase activity; regulation of the force of heart contraction by chemical signal; lipopolysaccharide-mediated signaling pathway; angiogenesis; negative regulation of blood pressure; regulation of blood vessel size; nitric oxide biosynthetic process; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; vascular endothelial growth factor receptor signaling pathway; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia

Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic

Research Articles on eNOS

1. study to determine the association between the eNOS 4a/b polymorphism and breast cancer in Mexican women; genotypes a/a-a/b are associated with breast cancer susceptibility in the analyzed samples from the Mexican population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with eNOS elisa kit

Products by Pathway	Pathway Diagram
ACE Inhibitor Pathway antibodies	ACE Inhibitor Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Angiogenesis Pathway antibodies	Angiogenesis Pathway Diagram
Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Endothelin Pathway antibodies	Endothelin Pathway Diagram

Diseases associated with eNOS elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>1908 publications with eNOS and Cardiovascular Diseases
Hypertension Antibodies	>943 publications with eNOS and Hypertension
Myocardial Ischemia Antibodies	>514 publications with eNOS and Myocardial Ischemia
Ischemia Antibodies	>492 publications with eNOS and Ischemia
Atherosclerosis Antibodies	>423 publications with eNOS and Atherosclerosis
Coronary Artery Disease Antibodies	>346 publications with eNOS and Coronary Artery Disease
Arteriosclerosis Antibodies	>321 publications with eNOS and Arteriosclerosis
Reperfusion Injury Antibodies	>247 publications with eNOS and Reperfusion Injury
Myocardial Infarction Antibodies	>222 publications with eNOS and Myocardial Infarction
Neoplasms Antibodies	>198 publications with eNOS and Neoplasms

Organs/Tissues associated with eNOS elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>3282 publications with eNOS and Vascular
Blood Antibodies	>2461 publications with eNOS and Blood
Heart Antibodies	>1134 publications with eNOS and Heart
Kidney Antibodies	>479 publications with eNOS and Kidney
Brain Antibodies	>405 publications with eNOS and Brain
Lung Antibodies	>347 publications with eNOS and Lung
Liver Antibodies	>242 publications with eNOS and Liver
Embryonic Tissue Antibodies	>199 publications with eNOS and Embryonic Tissue
Bone Antibodies	>129 publications with eNOS and Bone
Skin Antibodies	>90 publications with eNOS and Skin