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Free Fatty Acid (FFA) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS268756

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human FFA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details

$340 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Free Fatty Acid (FFA), ELISA Kit
Full Product Name
Human Free Fatty Acid (FFA) ELISA Kit
Product Gene Name
FFA elisa kit
Species Reactivity
Human
Assay Type
Sandwich
Detection Range
10 mmol/L-0.156 mmol/L
Sensitivity
Up to 0.05 mmol/L
Specificity
No cross-reaction with other factors.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
37,144 Da
OMIM
603823
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of FFA elisa kit

Human FFA ELISA Kit – Cat# MBS268756

Other Notes

Small volumes of FFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FFA purchase

MBS268756 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Free Fatty Acid (FFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FFA. The ELISA analytical biochemical technique of the MBS268756 kit is based on FFA antibody-FFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FFA. It may not necessarily be applicable to this product.

NCBI GI #
4885333
NCBI GeneID
2867
NCBI Accession #
NP_005297.1
UniProt Secondary Accession #
Q4VAZ3; Q4VAZ5; Q4VBL5; B0M0J9
UniProt Related Accession #
O15552
GenBank Nucleotide #
NM_005306.2
NCBI Official Full Name
free fatty acid receptor 2
NCBI Official Synonym Full Names
free fatty acid receptor 2
NCBI Official Symbol
FFAR2
NCBI Official Synonym Symbols
FFA2R; GPR43
UniProt Protein Name
Free fatty acid receptor 2
UniProt Synonym Protein Names
G-protein coupled receptor 43
UniProt Gene Name
FFAR2
UniProt Synonym Gene Names
GPR43
UniProt Entry Name
FFAR2_HUMAN
NCBI Protein Information
free fatty acid receptor 2; G protein-coupled receptor 43; G-protein coupled receptor 43; free fatty acid activated receptor 2

NCBI Summary for FFA

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]
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UniProt Comments for FFA

FFAR2: Receptor for short chain fatty acids through a G(i)- protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell projection; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding

Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; cell surface pattern recognition receptor signaling pathway; positive regulation of cytokine production during immune response; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production
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Research Articles on FFA

1. GPR43 modulates NF-kappaB activity via beta-arrestin 2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FFA elisa kit

 Reference Product  PubMed Publications
 FFAR1 elisa kit  >6 publications with FFA and FFAR1
 CCK elisa kit  >1 publications with FFA and CCK

Pathways associated with FFA elisa kit

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Free Fatty Acid Receptors Pathway antibodies  Free Fatty Acid Receptors Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram

Diseases associated with FFA elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with FFA and Inflammation
 Colitis Antibodies  >5 publications with FFA and Colitis
 Fatty Liver Antibodies  >4 publications with FFA and Fatty Liver
 Liver Diseases Antibodies  >2 publications with FFA and Liver Diseases
 Hyperglycemia Antibodies  >2 publications with FFA and Hyperglycemia
 Disease Models, Animal Antibodies  >2 publications with FFA and Disease Models, Animal
 Asthma Antibodies  >1 publications with FFA and Asthma
 Nervous System Diseases Antibodies  >1 publications with FFA and Nervous System Diseases
 Hypersensitivity Antibodies  >1 publications with FFA and Hypersensitivity
 Weight Loss Antibodies  >1 publications with FFA and Weight Loss

Organs/Tissues associated with FFA elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with FFA and Lung
 Pancreas Antibodies  >1 publications with FFA and Pancreas
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