Phosphorylated Smad 2 (pSmad2) ELISA Kit for Human

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human pSmad2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

Small volumes of pSmad2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS269933 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphorylated Smad 2 (pSmad2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing pSmad2. The ELISA analytical biochemical technique of the MBS269933 kit is based on pSmad2 antibody-pSmad2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect pSmad2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, pSmad2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). The SMAD family of transcription factors is phosphorylated by growth factors before transducing cellular signals to the nucleus. SMAD2 (Mothers against decapentaplegic homolog 2), similar to SMAD3, is serine phosphorylated by TGF beta superfamily receptors, initiating cell signaling. C-terminal SMAD2 ser465 467 and ser423 425, but not SMAD3 ser423 are readily detected by a phospho SMAD2 sandwich ELISA kit from Pathscanâ available through MyBiosource.com. These kits are designed for specific detection of phospho SMAD2 and not total SMAD2. No cross reaction with 467 SMAD3 protein or other related proteins is demonstrated, making this Pathscanâ® phospho 425 ELISA kit a bargain for signaling technology research. Available with precoated rabbit mAb 96 well plate, sample diluent, cell lysis buffer, wash solution, detection antibody HRP linked and stop solution, this product and the related Pathscanâ® total SMAD can be performed in as little as 30 min with accuracy in 5000 pg/ml-78 pg/ml ranges.