Phosphorylated Smad 3 (pSmad3) ELISA Kit for Human

Name: Human pSmad3 ELISA Kit; Phosphorylated Smad 3; MyBioSource
Brand: MyBioSource
SKU: MBS269936
Price: 340 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS269936

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human pSmad3 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details

$340 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phosphorylated Smad 3 (pSmad3), ELISA Kit

Full Product Name

Human Phosphorylated Smad 3 (pSmad3) ELISA Kit

Product Gene Name

pSmad3 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Detection Range

5000 pg/mL-78 pg/mL

Sensitivity

Up to 20 pg/mL

Specificity

No cross-reaction with other factors.

Preparation and Storage

Store all reagents at 2-8 degree C.

Inter-assay Precision

<= 12%

Intra-assay Precision

<= 8%

Molecular Weight

42,417 Da

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of pSmad3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for pSmad3 purchase

MBS269936 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphorylated Smad 3 (pSmad3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing pSmad3. The ELISA analytical biochemical technique of the MBS269936 kit is based on pSmad3 antibody-pSmad3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect pSmad3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, pSmad3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for pSmad3. It may not necessarily be applicable to this product.

NCBI GI #

8394012

NCBI GeneID

24666

NCBI Accession #

NP_058734.1

UniProt Related Accession #

P20650

GenBank Nucleotide #

NM_017038.1

NCBI Official Full Name

protein phosphatase 1A

NCBI Official Synonym Full Names

protein phosphatase, Mg2+/Mn2+ dependent, 1A

NCBI Official Symbol

Ppm1a

NCBI Official Synonym Symbols

Pp2c1

UniProt Protein Name

Protein phosphatase 1A

UniProt Synonym Protein Names

Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA

UniProt Gene Name

Ppm1a

UniProt Synonym Gene Names

Pp2c1; Pppm1a; PP2C-alpha

UniProt Entry Name

PPM1A_RAT

NCBI Protein Information

protein phosphatase 1A; IA; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha; protein phosphatase 1A, magnesium dependent, alpha isoform; Protein phosphatase type 1A (formely 2C) Mg-dependent alpha isoform; Protein phosphatase type 1A (formely 2C), Mg-dependent, alpha isoform

UniProt Comments for pSmad3

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Cellular Component: neuron projection; membrane; voltage-gated calcium channel complex; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: positive regulation of protein export from nucleus; N-terminal protein myristoylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; dephosphorylation; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transforming growth factor beta receptor signaling pathway; protein amino acid dephosphorylation; positive regulation of Wnt receptor signaling pathway; negative regulation of BMP signaling pathway

Research Articles on pSmad3

1. Maxacalcitol ameliorates tubulointerstitial fibrosis in obstructed kidneys by recruiting PPM1A/VDR complex to pSmad3. This recruitment promoted the dephosphorylation of pSmad3 and attenuated the pSmad3-dependent production of TGF-beta1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with pSmad3 elisa kit

Reference Product	PubMed Publications
Smad2 elisa kit	>13 publications with pSmad3 and Smad2
Smad3 elisa kit	>10 publications with pSmad3 and Smad3
Smad4 elisa kit	>1 publications with pSmad3 and Smad4

Pathways associated with pSmad3 elisa kit

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway antibodies	Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway Diagram
Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies	Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
IGF1R Signaling Cascade Pathway antibodies	IGF1R Signaling Cascade Pathway Diagram
IRS-mediated Signalling Pathway antibodies	IRS-mediated Signalling Pathway Diagram
IRS-related Events Pathway antibodies	IRS-related Events Pathway Diagram
IRS-related Events Triggered By IGF1R Pathway antibodies	IRS-related Events Triggered By IGF1R Pathway Diagram
Insulin Receptor Signalling Cascade Pathway antibodies	Insulin Receptor Signalling Cascade Pathway Diagram

Diseases associated with pSmad3 elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>5 publications with pSmad3 and Necrosis
Fibrosis Antibodies	>4 publications with pSmad3 and Fibrosis
Disease Models, Animal Antibodies	>2 publications with pSmad3 and Disease Models, Animal
Kidney Diseases Antibodies	>2 publications with pSmad3 and Kidney Diseases
Adenocarcinoma Antibodies	>1 publications with pSmad3 and Adenocarcinoma
Depressive Disorder Antibodies	>1 publications with pSmad3 and Depressive Disorder
Neoplasm Metastasis Antibodies	>1 publications with pSmad3 and Neoplasm Metastasis
Carcinoma, Hepatocellular Antibodies	>1 publications with pSmad3 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with pSmad3 and Liver Neoplasms
Inflammation Antibodies	>1 publications with pSmad3 and Inflammation

Organs/Tissues associated with pSmad3 elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>4 publications with pSmad3 and Embryonic Tissue
Liver Antibodies	>2 publications with pSmad3 and Liver
Eye Antibodies	>2 publications with pSmad3 and Eye
Kidney Antibodies	>2 publications with pSmad3 and Kidney
Connective Tissue Antibodies	>2 publications with pSmad3 and Connective Tissue
Placenta Antibodies	>2 publications with pSmad3 and Placenta
Brain Antibodies	>2 publications with pSmad3 and Brain
Ovary Antibodies	>1 publications with pSmad3 and Ovary