Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit for Porcine

Name: Porcine AP-1 ELISA Kit; Transcription Factor / Activator Protein 1; MyBioSource
Brand: MyBioSource
SKU: MBS269963
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS269963

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine AP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and… More details

$370 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Transcription Factor / Activator Protein 1 (AP-1), ELISA Kit

Full Product Name

Porcine Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

Product Gene Name

AP-1 elisa kit

Species Reactivity

Porcine

Assay Type

Sandwich

Samples

Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AP-1 concentration

Detection Range

10 ng/ml-0.156ng/ml

Sensitivity

0.05 ng/ml.

Specificity

No cross-reaction with other factors.

Preparation and Storage

Store all reagents at 2-8 degree C.

Inter-assay Precision

<= 12%

Intra-assay Precision

<= 8%

Molecular Weight

35,879 Da

OMIM

165161

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of AP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AP-1 purchase

MBS269963 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AP-1. The ELISA analytical biochemical technique of the MBS269963 kit is based on AP-1 antibody-AP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AP-1. It may not necessarily be applicable to this product.

NCBI GI #

4504809

NCBI GeneID

3726

NCBI Accession #

NP_002220.1

UniProt Secondary Accession #

Q96GH3

UniProt Related Accession #

P17275

GenBank Nucleotide #

NM_002229.2

NCBI Official Full Name

transcription factor jun-B

NCBI Official Synonym Full Names

jun B proto-oncogene

NCBI Official Symbol

JUNB

NCBI Official Synonym Symbols

AP-1

UniProt Protein Name

Transcription factor jun-B

UniProt Gene Name

JUNB

UniProt Entry Name

JUNB_HUMAN

NCBI Protein Information

transcription factor jun-B; activator protein 1

UniProt Comments for AP-1

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: response to drug; response to peptide hormone stimulus; response to light stimulus; transcription initiation from RNA polymerase II promoter; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; trophectodermal cell differentiation; cellular response to hormone stimulus; osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; positive regulation of cell differentiation; vasculogenesis; response to corticosterone stimulus

Research Articles on AP-1

1. These results demonstrate a unique induction of JUNB in response to kinase inhibitor therapies that may be among the earliest events in the progression to treatment resistance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AP-1 elisa kit

Reference Product	PubMed Publications
FOS elisa kit	>704 publications with AP-1 and FOS
FOSB elisa kit	>204 publications with AP-1 and FOSB
FOSL2 elisa kit	>35 publications with AP-1 and FOSL2
SMAD4 elisa kit	>15 publications with AP-1 and SMAD4
IL6 elisa kit	>14 publications with AP-1 and IL6
MAPK8 elisa kit	>6 publications with AP-1 and MAPK8
FOSL1 elisa kit	>4 publications with AP-1 and FOSL1
EP300 elisa kit	>3 publications with AP-1 and EP300
BCL6 elisa kit	>2 publications with AP-1 and BCL6

Pathways associated with AP-1 elisa kit

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Corticotropin-releasing Hormone Pathway antibodies	Corticotropin-releasing Hormone Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
IL6-mediated Signaling Events Pathway antibodies	IL6-mediated Signaling Events Pathway Diagram
Integrated Pancreatic Cancer Pathway antibodies	Integrated Pancreatic Cancer Pathway Diagram

Diseases associated with AP-1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>81 publications with AP-1 and Inflammation
Necrosis Antibodies	>68 publications with AP-1 and Necrosis
Nervous System Diseases Antibodies	>51 publications with AP-1 and Nervous System Diseases
Disease Models, Animal Antibodies	>47 publications with AP-1 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>44 publications with AP-1 and Cardiovascular Diseases
Liver Diseases Antibodies	>37 publications with AP-1 and Liver Diseases
Brain Diseases Antibodies	>35 publications with AP-1 and Brain Diseases
Neoplasms, Experimental Antibodies	>34 publications with AP-1 and Neoplasms, Experimental
Liver Neoplasms Antibodies	>31 publications with AP-1 and Liver Neoplasms
Lung Diseases Antibodies	>25 publications with AP-1 and Lung Diseases

Organs/Tissues associated with AP-1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>209 publications with AP-1 and Blood
Brain Antibodies	>182 publications with AP-1 and Brain
Bone Antibodies	>84 publications with AP-1 and Bone
Liver Antibodies	>83 publications with AP-1 and Liver
Skin Antibodies	>76 publications with AP-1 and Skin
Nerve Antibodies	>59 publications with AP-1 and Nerve
Lung Antibodies	>56 publications with AP-1 and Lung
Vascular Antibodies	>56 publications with AP-1 and Vascular
Bone Marrow Antibodies	>40 publications with AP-1 and Bone Marrow
Kidney Antibodies	>33 publications with AP-1 and Kidney