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Proteinase K Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS278041

Proteinase K is a protease with serine as its active center. It has broad substrate specificity, and it preferentially decomposes the ester and peptide bonds of the C-terminus of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Proteinase K, Recombinant Protein
Full Product Name
Proteinase K
Host
Tritirachium album limber
Form/Format
Lyophilized powder
Purity/Purification
Over 99% (Native PAGE and SDS PAGE assay), Molecular Biology Grade, RNase/DNase free
Preparation and Storage
Room temperature (15-30 degree C)
Molecular Weight
18.5 kD
DNAses and RNAses
Not Detected
Protocol and Precautions
1. Add 5 ml Proteinase K Storage Buffer to 100 mg Proteinase K and mix well to completely dissolve Proteinase K. The final concentration will be 20 mg/ml.
2. Working concentrations of the enzyme range from 50–250 ug/ml. Working temperature is 25 degree C ~ 65 degree C. Ideal temperature is 58 degree C, incubation time is 15 minutes to 48 hours. The exact time of incubation and Proteinase K concentration depends on the amount of protein in the sample.
Note: Dissolved Proteinase K should not be placed at room temperature for a long time. Avoid repeated freezing and thawing to reduce its activity. -20 degree C for long-term storage.

Other Notes

Small volumes of Proteinase K recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Proteinase K purchase

MBS278041 is a recombinant protein targeting Proteinase K. Host: Tritirachium album limber.

Additional Details

NCBI/Uniprot data below describe general gene information for Proteinase K. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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