Proteinase-Activated Receptor 3 (F2RL2) ELISA Kit for Human

Name: Human F2RL2 ELISA Kit; Proteinase-Activated Receptor 3; MyBioSource
Brand: MyBioSource
SKU: MBS280143
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280143

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate F2RL2 in samples. An antibody specific for F2RL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF2RL2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Proteinase-activated receptor 3 (F2RL2), ELISA Kit

Full Product Name

Human Proteinase-activated receptor 3 (F2RL2) ELISA Kit

Product Gene Name

F2RL2 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human F2RL2. No significant cross-reactivity or interference between Human F2RL2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

40,247 Da

OMIM

U92971 mRNA

3D Structure

ModBase 3D Structure for O00254

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of F2RL2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F2RL2 purchase

MBS280143 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proteinase-activated receptor 3 (F2RL2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F2RL2. The ELISA analytical biochemical technique of the MBS280143 kit is based on F2RL2 antibody-F2RL2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F2RL2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F2RL2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for F2RL2. It may not necessarily be applicable to this product.

NCBI GI #

375151568

NCBI GeneID

2151

NCBI Accession #

NP_001243495.1

UniProt Primary Accession #

O00254

UniProt Secondary Accession #

Q52M68; Q7Z3W3; B2R754; B4DQ13

UniProt Related Accession #

O00254

GenBank Nucleotide #

NM_001256566.1

NCBI Official Full Name

proteinase-activated receptor 3 isoform 2

NCBI Official Synonym Full Names

coagulation factor II thrombin receptor like 2

NCBI Official Symbol

F2RL2

NCBI Official Synonym Symbols

PAR3; PAR-3

UniProt Protein Name

Proteinase-activated receptor 3

UniProt Synonym Protein Names

Coagulation factor II receptor-like 2; Thrombin receptor-like 2

Protein Family

Proteinase-activated receptor

UniProt Gene Name

F2RL2

UniProt Synonym Gene Names

PAR3; PAR-3

NCBI Protein Information

proteinase-activated receptor 3

NCBI Summary for F2RL2

This gene encodes a member of the protease-activated receptor (PAR) family which is a subfamily of the seven transmembrane G protein-coupled cell surface receptor family. The encoded protein acts as a cofactor in the thrombin-mediated cleavage and activation of the protease-activated receptor family member PAR4. The encoded protein plays an essential role in hemostasis and thrombosis. Alternate splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Feb 2012]

UniProt Comments for F2RL2

PAR3: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Interacts with INSC/inscuteable and probably GPSM2. Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: apical plasma membrane; extracellular region; integral component of plasma membrane; plasma membrane; protein complex

Molecular Function: G-protein coupled receptor activity; phosphoinositide phospholipase C activity; protein binding; thrombin receptor activity

Biological Process: blood coagulation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein coupled receptor signaling pathway; platelet activation; positive regulation of Rho protein signal transduction; response to wounding

Research Articles on F2RL2

1. PAR3 is essential in thrombin stimulated insulin secretion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with F2RL2 elisa kit

Reference Product	PubMed Publications
F2RL3 elisa kit	>1 publications with F2RL2 and F2RL3

Pathways associated with F2RL2 elisa kit

Products by Pathway	Pathway Diagram
CDC42 Signaling Events Pathway antibodies	CDC42 Signaling Events Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
ErbB1 Downstream Signaling Pathway antibodies	ErbB1 Downstream Signaling Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Insulin Pathway antibodies	Insulin Pathway Diagram

Diseases associated with F2RL2 elisa kit

Disease Name	Pubmed Publications
Drug Toxicity Antibodies	>1 publications with F2RL2 and Drug Toxicity
Inflammation Antibodies	>1 publications with F2RL2 and Inflammation

Organs/Tissues associated with F2RL2 elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>1 publications with F2RL2 and Vascular
Muscle Antibodies	>1 publications with F2RL2 and Muscle