V-Type Proton Atpase 16 Kda Proteolipid Subunit (ATP6V0C) ELISA Kit for Sheep

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ATP6V0C in samples. An antibody specific for ATP6V0C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATP6V0C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP6V0C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP6V0C bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ATP6V0C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280153 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V-type proton ATPase 16 kDa proteolipid subunit (ATP6V0C) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP6V0C. The ELISA analytical biochemical technique of the MBS280153 kit is based on ATP6V0C antibody-ATP6V0C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP6V0C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP6V0C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).