Polycomb Group RING Finger Protein 1 (PCGF1) ELISA Kit for Human

Name: Human PCGF1 ELISA Kit; Polycomb Group RING Finger Protein 1; MyBioSource
Brand: MyBioSource
SKU: MBS280196
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280196

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PCGF1 in Human serum, plasma, tissue homogenates. An antibody specific for PCGF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Polycomb group RING finger protein 1 (PCGF1), ELISA Kit

Full Product Name

Human Polycomb group RING finger protein 1 (PCGF1) ELISA Kit

Product Gene Name

PCGF1 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

0.156 ng/mL - 10 ng/mL

Sensitivity

0.1 ng/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PCGF1. No significant cross-reactivity or interference between Human PCGF1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

20,904 Da

OMIM

AF087884 mRNA

3D Structure

ModBase 3D Structure for Q9BSM1

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PCGF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCGF1 purchase

MBS280196 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Polycomb group RING finger protein 1 (PCGF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PCGF1. The ELISA analytical biochemical technique of the MBS280196 kit is based on PCGF1 antibody-PCGF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCGF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCGF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PCGF1. It may not necessarily be applicable to this product.

NCBI GI #

109240538

NCBI GeneID

84759

NCBI Accession #

NP_116062.2

UniProt Primary Accession #

Q9BSM1

UniProt Secondary Accession #

Q7Z506

UniProt Related Accession #

Q9BSM1

GenBank Nucleotide #

NM_032673.2

NCBI Official Full Name

polycomb group RING finger protein 1

NCBI Official Synonym Full Names

polycomb group ring finger 1

NCBI Official Symbol

PCGF1

NCBI Official Synonym Symbols

NSPC1; RNF68; RNF3A-2; 2010002K04Rik

UniProt Protein Name

Polycomb group RING finger protein 1

UniProt Synonym Protein Names

Nervous system Polycomb-1; NSPc1; RING finger protein 68

Protein Family

Polycomb group RING finger protein

UniProt Gene Name

PCGF1

UniProt Synonym Gene Names

NSPC1; RNF68; NSPc1

NCBI Protein Information

polycomb group RING finger protein 1

NCBI Summary for PCGF1

PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM, Aug 2008]

UniProt Comments for PCGF1

PCGF1: Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin- dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down- regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: nucleoplasm; nucleus; PcG protein complex

Molecular Function: metal ion binding; protein binding; protein C-terminus binding

Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent

Research Articles on PCGF1

1. these results demonstrate that NSPc1 promotes cancer stem cell self-renewal by repressing the synthesis of all-trans retinoic acid via targeting RDH16 and may provide novel targets for glioma treatment in the future.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PCGF1 elisa kit

Reference Product	PubMed Publications
BCOR elisa kit	>4 publications with PCGF1 and BCOR
RNF2 elisa kit	>4 publications with PCGF1 and RNF2
RING1 elisa kit	>2 publications with PCGF1 and RING1
CBX2 elisa kit	>1 publications with PCGF1 and CBX2
EZH2 elisa kit	>1 publications with PCGF1 and EZH2
PCGF5 elisa kit	>1 publications with PCGF1 and PCGF5
CBX4 elisa kit	>1 publications with PCGF1 and CBX4
BAP1 elisa kit	>1 publications with PCGF1 and BAP1

Pathways associated with PCGF1 elisa kit

Products by Pathway	Pathway Diagram
Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies	Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies	Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram

Diseases associated with PCGF1 elisa kit

Disease Name	Pubmed Publications
Carcinoma, Hepatocellular Antibodies	>1 publications with PCGF1 and Carcinoma, Hepatocellular
Inflammation Antibodies	>1 publications with PCGF1 and Inflammation
Necrosis Antibodies	>1 publications with PCGF1 and Necrosis

Organs/Tissues associated with PCGF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Ascites Antibodies	>1 publications with PCGF1 and Ascites
Placenta Antibodies	>1 publications with PCGF1 and Placenta
Eye Antibodies	>1 publications with PCGF1 and Eye