Protein-Lysine 6-Oxidase (LOX) ELISA Kit for Human

Name: Human LOX ELISA Kit; Protein-Lysine 6-Oxidase; MyBioSource
Brand: MyBioSource
SKU: MBS280253
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280253

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate INS in Sheep serum, plasma. An antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INS present is bound… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Protein-lysine 6-oxidase (LOX), ELISA Kit

Full Product Name

Human Protein-lysine 6-oxidase (LOX) ELISA Kit

Product Gene Name

LOX elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma And Other Biological Fluids

Detection Range

0.31 ng/mL-20 ng/mL

Sensitivity

0.1 ng/mL (mean of 6 independent assays).

Specificity

This assay has high sensitivity and excellent specificity for detection of Sheep INS. No significant cross-reactivity or interference between Sheep INS and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

46,944 Da

OMIM

S78694 mRNA

3D Structure

ModBase 3D Structure for P28300

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of LOX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LOX purchase

MBS280253 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein-lysine 6-oxidase (LOX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LOX. The ELISA analytical biochemical technique of the MBS280253 kit is based on LOX antibody-LOX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LOX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LOX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LOX. It may not necessarily be applicable to this product.

NCBI GI #

20149540

NCBI GeneID

4015

NCBI Accession #

NP_002308.2

UniProt Primary Accession #

P28300

UniProt Secondary Accession #

Q5FWF0; B2R5Q3

UniProt Related Accession #

P28300

GenBank Nucleotide #

NM_002317.6

NCBI Official Full Name

protein-lysine 6-oxidase isoform 1 preproprotein

NCBI Official Synonym Full Names

lysyl oxidase

NCBI Official Symbol

LOX

NCBI Official Synonym Symbols

AAT10

UniProt Protein Name

Protein-lysine 6-oxidase

UniProt Synonym Protein Names

Lysyl oxidase

Protein Family

Linoleate 9/13-lipoxygenase

UniProt Gene Name

LOX

NCBI Protein Information

protein-lysine 6-oxidase

NCBI Summary for LOX

This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. In addition, defects in this gene have been linked with predisposition to thoracic aortic aneurysms and dissections. [provided by RefSeq, Jul 2016]

UniProt Comments for LOX

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: EC 1.4.3.13; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q23.1

Cellular Component: collagen; extracellular region; extracellular space; nucleus; proteinaceous extracellular matrix

Molecular Function: copper ion binding; protein binding; protein-lysine 6-oxidase activity

Biological Process: blood vessel morphogenesis; cellular protein modification process; collagen fibril organization; elastic fiber assembly; extracellular matrix organization; heart development; lung development; response to drug; response to steroid hormone; wound healing

Disease: Aortic Aneurysm, Familial Thoracic 10

Research Articles on LOX

1. Our findings suggest that LOX has a role in cancer cell mitosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LOX elisa kit

Reference Product	PubMed Publications
LOXL1 elisa kit	>63 publications with LOX and LOXL1
SRC elisa kit	>37 publications with LOX and SRC
FGF2 elisa kit	>15 publications with LOX and FGF2
BMP1 elisa kit	>8 publications with LOX and BMP1
FBLN5 elisa kit	>8 publications with LOX and FBLN5
SNAI1 elisa kit	>2 publications with LOX and SNAI1
EFEMP2 elisa kit	>2 publications with LOX and EFEMP2

Pathways associated with LOX elisa kit

Products by Pathway	Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Crosslinking Of Collagen Fibrils Pathway antibodies	Crosslinking Of Collagen Fibrils Pathway Diagram
Elastic Fibre Formation Pathway antibodies	Elastic Fibre Formation Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram

Diseases associated with LOX elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>672 publications with LOX and Inflammation
Cardiovascular Diseases Antibodies	>628 publications with LOX and Cardiovascular Diseases
Disease Models, Animal Antibodies	>424 publications with LOX and Disease Models, Animal
Necrosis Antibodies	>255 publications with LOX and Necrosis
Heart Diseases Antibodies	>194 publications with LOX and Heart Diseases
Lung Diseases Antibodies	>179 publications with LOX and Lung Diseases
Neoplasms, Experimental Antibodies	>158 publications with LOX and Neoplasms, Experimental
Hypertension Antibodies	>155 publications with LOX and Hypertension
Fibrosis Antibodies	>155 publications with LOX and Fibrosis
Lung Neoplasms Antibodies	>132 publications with LOX and Lung Neoplasms

Organs/Tissues associated with LOX elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>780 publications with LOX and Vascular
Muscle Antibodies	>426 publications with LOX and Muscle
Brain Antibodies	>398 publications with LOX and Brain
Lung Antibodies	>305 publications with LOX and Lung
Heart Antibodies	>290 publications with LOX and Heart
Liver Antibodies	>263 publications with LOX and Liver
Bone Antibodies	>250 publications with LOX and Bone
Skin Antibodies	>187 publications with LOX and Skin
Kidney Antibodies	>175 publications with LOX and Kidney
Embryonic Tissue Antibodies	>164 publications with LOX and Embryonic Tissue