Zinc Finger E-Box-Binding Homeobox 1 (ZEB1) ELISA Kit for Mouse

Name: Mouse ZEB1 ELISA Kit; Zinc Finger E-Box-Binding Homeobox 1; MyBioSource
Brand: MyBioSource
SKU: MBS280255
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280255

TCF8 encodes a human zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.Delta-Ef1 selectively transactivated the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Zinc finger E-box-binding homeobox 1 (ZEB1), ELISA Kit

Full Product Name

Mouse Zinc finger E-box-binding homeobox 1 (ZEB1) ELISA Kit

Product Gene Name

ZEB1 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse ZEB1. No significant cross-reactivity or interference between Mouse ZEB1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

122,465 Da

3D Structure

ModBase 3D Structure for Q64318

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ZEB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZEB1 purchase

MBS280255 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger E-box-binding homeobox 1 (ZEB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZEB1. The ELISA analytical biochemical technique of the MBS280255 kit is based on ZEB1 antibody-ZEB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZEB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZEB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ZEB1. It may not necessarily be applicable to this product.

NCBI GI #

6756073

NCBI GeneID

21417

NCBI Accession #

NP_035676.1

UniProt Primary Accession #

Q64318

UniProt Secondary Accession #

Q62519; A4QPD2

UniProt Related Accession #

Q64318

GenBank Nucleotide #

NM_011546.3

NCBI Official Full Name

zinc finger E-box-binding homeobox 1

NCBI Official Synonym Full Names

zinc finger E-box binding homeobox 1

NCBI Official Symbol

Zeb1

NCBI Official Synonym Symbols

Tw; BZP; ZEB; MEB1; Nil2; Tcf8; AREB6; TCF-8; Tcf18; Zfhep; Zfx1a; Zfhx1a; Zfx1ha; [delta]EF1; 3110032K11Rik

UniProt Protein Name

Zinc finger E-box-binding homeobox 1

UniProt Synonym Protein Names

Delta EF1; Transcription factor 8; TCF-8; Zinc finger homeobox protein 1a; MEB1

Protein Family

Zinc finger E-box-binding homeobox

UniProt Gene Name

Zeb1

UniProt Synonym Gene Names

Tcf8; Zfhx1a; Zfx1a; Zfx1ha; TCF-8; MEB1

NCBI Protein Information

zinc finger E-box-binding homeobox 1

UniProt Comments for ZEB1

TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 18 4.42 cM|18 A1

Cellular Component: cytosol; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; metal ion binding; nucleic acid binding; protein binding; sequence-specific DNA binding; transcription factor binding

Biological Process: animal organ development; cartilage development; cell differentiation; central nervous system development; cochlea morphogenesis; embryonic camera-type eye morphogenesis; embryonic morphogenesis; embryonic skeletal morphogenesis; negative regulation of cell proliferation; negative regulation of endothelial cell differentiation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; pattern specification process; positive regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; regulation of smooth muscle cell differentiation; regulation of T cell differentiation in the thymus; regulation of transcription, DNA-templated; regulation of transforming growth factor beta receptor signaling pathway; semicircular canal morphogenesis; transcription, DNA-dependent

Research Articles on ZEB1

1. These data establish ZEB1 as a key factor in the tumor microenvironment and for maintaining tumor-associated macrophages' tumor-promoting functions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ZEB1 elisa kit

Reference Product	PubMed Publications
Cdh1 elisa kit	>17 publications with ZEB1 and Cdh1
Ctbp2 elisa kit	>3 publications with ZEB1 and Ctbp2
Ctbp1 elisa kit	>3 publications with ZEB1 and Ctbp1

Pathways associated with ZEB1 elisa kit

Products by Pathway	Pathway Diagram
Hypertrophy Model Pathway antibodies	Hypertrophy Model Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram
TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with ZEB1 elisa kit

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>123 publications with ZEB1 and Neoplasm Metastasis
Neoplasm Invasiveness Antibodies	>106 publications with ZEB1 and Neoplasm Invasiveness
Breast Neoplasms Antibodies	>94 publications with ZEB1 and Breast Neoplasms
Skin Diseases Antibodies	>86 publications with ZEB1 and Skin Diseases
Adenocarcinoma Antibodies	>76 publications with ZEB1 and Adenocarcinoma
Disease Progression Antibodies	>68 publications with ZEB1 and Disease Progression
Lung Diseases Antibodies	>44 publications with ZEB1 and Lung Diseases
Cell Transformation, Neoplastic Antibodies	>37 publications with ZEB1 and Cell Transformation, Neoplastic
Fibrosis Antibodies	>28 publications with ZEB1 and Fibrosis
Inflammation Antibodies	>26 publications with ZEB1 and Inflammation

Organs/Tissues associated with ZEB1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>12 publications with ZEB1 and Brain
Thymus Antibodies	>2 publications with ZEB1 and Thymus