Activated RNA Polymerase II Transcriptional Coactivator P15 (SUB1) ELISA Kit for Rat

Name: Rat SUB1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS280319
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280319

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SUB1 in samples. An antibody specific for SUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySUB1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Activated RNA polymerase II transcriptional coactivator p15 (SUB1), ELISA Kit

Full Product Name

Rat Activated RNA polymerase II transcriptional coactivator p15 (SUB1) ELISA Kit

Product Gene Name

SUB1 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat SUB1. No significant cross-reactivity or interference between Rat SUB1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

14,441 Da

3D Structure

ModBase 3D Structure for Q63396

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SUB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SUB1 purchase

MBS280319 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activated RNA polymerase II transcriptional coactivator p15 (SUB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SUB1. The ELISA analytical biochemical technique of the MBS280319 kit is based on SUB1 antibody-SUB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SUB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SUB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SUB1. It may not necessarily be applicable to this product.

NCBI GI #

57526804

NCBI GeneID

192269

NCBI Accession #

NP_001009618.1

UniProt Primary Accession #

Q63396

UniProt Secondary Accession #

Q5M805

UniProt Related Accession #

Q63396

GenBank Nucleotide #

NM_001009618.1

NCBI Official Full Name

activated RNA polymerase II transcriptional coactivator p15

NCBI Official Synonym Full Names

SUB1 homolog, transcriptional regulator

NCBI Official Symbol

Sub1

NCBI Official Synonym Symbols

Rpo2tc1

UniProt Protein Name

Activated RNA polymerase II transcriptional coactivator p15

UniProt Synonym Protein Names

Positive cofactor 4; PC4; SUB1 homolog; p14

Protein Family

Activated RNA polymerase II transcriptional coactivator

UniProt Gene Name

Sub1

UniProt Synonym Gene Names

Pc4; Rpo2tc1; PC4

NCBI Protein Information

activated RNA polymerase II transcriptional coactivator p15

NCBI Summary for SUB1

expressed in 12 day embryo and in a chemically induced pancreatic B-cell tumor; not expressed in normal differentiated cells [RGD, Feb 2006]

UniProt Comments for SUB1

PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q16

Cellular Component: nucleolus; nucleus; transcription factor complex

Molecular Function: activating transcription factor binding; DNA helicase activity; double-stranded DNA binding; identical protein binding; RNA binding; single-stranded DNA binding; transcription coactivator activity

Biological Process: DNA duplex unwinding; protein homooligomerization; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with SUB1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6 publications with SUB1 and Neoplasms
Breast Neoplasms Antibodies	>4 publications with SUB1 and Breast Neoplasms
Skin Diseases Antibodies	>3 publications with SUB1 and Skin Diseases
Lung Neoplasms Antibodies	>2 publications with SUB1 and Lung Neoplasms
Colorectal Neoplasms Antibodies	>1 publications with SUB1 and Colorectal Neoplasms
Substance Withdrawal Syndrome Antibodies	>1 publications with SUB1 and Substance Withdrawal Syndrome
Nervous System Diseases Antibodies	>1 publications with SUB1 and Nervous System Diseases
Adenocarcinoma Antibodies	>1 publications with SUB1 and Adenocarcinoma
Necrosis Antibodies	>1 publications with SUB1 and Necrosis
Drug Hypersensitivity Antibodies	>1 publications with SUB1 and Drug Hypersensitivity

Organs/Tissues associated with SUB1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with SUB1 and Brain
Lung Antibodies	>2 publications with SUB1 and Lung
Heart Antibodies	>1 publications with SUB1 and Heart
Colon Antibodies	>1 publications with SUB1 and Colon
Kidney Antibodies	>1 publications with SUB1 and Kidney
Eye Antibodies	>1 publications with SUB1 and Eye
Thymus Antibodies	>1 publications with SUB1 and Thymus
Spleen Antibodies	>1 publications with SUB1 and Spleen