Epididymal Secretory Protein E1 (NPC2) ELISA Kit for Pig

Name: Pig NPC2 ELISA Kit; Epididymal Secretory Protein E1; MyBioSource
Brand: MyBioSource
SKU: MBS280371
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280371

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NPC2 in samples. An antibody specific for NPC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPC2 present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Epididymal secretory protein E1 (NPC2), ELISA Kit

Full Product Name

Pig Epididymal secretory protein E1 (NPC2) ELISA Kit

Product Gene Name

NPC2 elisa kit

Species Reactivity

Pig

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Pig NPC2. No significant cross-reactivity or interference between Pig NPC2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

16,288 Da

3D Structure

ModBase 3D Structure for O97763

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NPC2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPC2 purchase

MBS280371 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epididymal secretory protein E1 (NPC2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPC2. The ELISA analytical biochemical technique of the MBS280371 kit is based on NPC2 antibody-NPC2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPC2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPC2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NPC2. It may not necessarily be applicable to this product.

NCBI GI #

47523496

NCBI GeneID

397410

NCBI Accession #

NP_999371.1

UniProt Primary Accession #

O97763

UniProt Related Accession #

O97763

GenBank Nucleotide #

NM_214206.1

NCBI Official Full Name

epididymal secretory protein E1

NCBI Official Symbol

NPC2

UniProt Protein Name

Epididymal secretory protein E1

UniProt Synonym Protein Names

16 kDa secretory protein; Niemann Pick type C2 protein homolog

Protein Family

Epididymal secretory protein

UniProt Gene Name

NPC2

NCBI Protein Information

epididymal secretory protein E1

UniProt Comments for NPC2

Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (). May be involved in the regulation of the lipid composition of sperm membranes during the maturation in the epididymis. Binds cholesterol in a 1:1 ratio.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NPC2 elisa kit

Products by Pathway	Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram

Diseases associated with NPC2 elisa kit

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>120 publications with NPC2 and Brain Diseases
Death Antibodies	>26 publications with NPC2 and Death
Disease Models, Animal Antibodies	>23 publications with NPC2 and Disease Models, Animal
Liver Diseases Antibodies	>9 publications with NPC2 and Liver Diseases
Cardiovascular Diseases Antibodies	>9 publications with NPC2 and Cardiovascular Diseases
Fatty Liver Antibodies	>7 publications with NPC2 and Fatty Liver
Nerve Degeneration Antibodies	>6 publications with NPC2 and Nerve Degeneration
Seizures Antibodies	>6 publications with NPC2 and Seizures
Atrophy Antibodies	>6 publications with NPC2 and Atrophy
Inflammation Antibodies	>6 publications with NPC2 and Inflammation

Organs/Tissues associated with NPC2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>52 publications with NPC2 and Brain
Liver Antibodies	>40 publications with NPC2 and Liver
Blood Antibodies	>14 publications with NPC2 and Blood
Lung Antibodies	>13 publications with NPC2 and Lung
Spleen Antibodies	>12 publications with NPC2 and Spleen
Skin Antibodies	>10 publications with NPC2 and Skin
Muscle Antibodies	>7 publications with NPC2 and Muscle
Ovary Antibodies	>6 publications with NPC2 and Ovary
Heart Antibodies	>3 publications with NPC2 and Heart
Testis Antibodies	>3 publications with NPC2 and Testis