F-Box Only Protein 32 (FBXO32) ELISA Kit for Pig

FBXO32爀ncodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FBXO32 in samples. An antibody specific for FBXO32 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFBXO32 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FBXO32 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FBXO32 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of FBXO32 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280436 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the F-box only protein 32 (FBXO32) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FBXO32. The ELISA analytical biochemical technique of the MBS280436 kit is based on FBXO32 antibody-FBXO32 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FBXO32 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FBXO32. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).