Poly (U)-Binding-Splicing Factor PUF60 (PUF60) ELISA Kit for Human

Name: Human PUF60 ELISA Kit; Poly (U)-Binding-Splicing Factor PUF60; MyBioSource
Brand: MyBioSource
SKU: MBS280488
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280488

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PUF60 in Human serum, plasma, tissue homogenates. An antibody specific for PUF60 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Poly (U)-binding-splicing factor PUF60, ELISA Kit

Full Product Name

Human Poly (U)-binding-splicing factor PUF60 (PUF60) ELISA Kit

Product Gene Name

PUF60 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

31.25 pg/mL - 2000 pg/mL

Sensitivity

15.6 pg/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PUF60. No significant cross-reactivity or interference between Human PUF60 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

55,399 Da

OMIM

AF217197 mRNA

3D Structure

ModBase 3D Structure for Q9UHX1

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PUF60 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PUF60 purchase

MBS280488 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Poly (U)-binding-splicing factor PUF60, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PUF60. The ELISA analytical biochemical technique of the MBS280488 kit is based on PUF60 antibody-PUF60 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PUF60 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PUF60. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PUF60. It may not necessarily be applicable to this product.

NCBI GI #

209862867

NCBI GeneID

22827

NCBI Accession #

NP_001129505.1

UniProt Primary Accession #

Q9UHX1

UniProt Secondary Accession #

Q969E7; Q96D94; Q96H63; Q99628; Q9NZA0; Q9UJY7; A8K8K8

UniProt Related Accession #

Q9UHX1

GenBank Nucleotide #

NM_001136033.2

NCBI Official Full Name

poly(U)-binding-splicing factor PUF60 isoform c

NCBI Official Synonym Full Names

poly(U) binding splicing factor 60

NCBI Official Symbol

PUF60

NCBI Official Synonym Symbols

FIR; VRJS; RoBPI; SIAHBP1

UniProt Protein Name

Poly(U)-binding-splicing factor PUF60

UniProt Synonym Protein Names

60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1

Protein Family

Poly(U)-binding-splicing factor

UniProt Gene Name

PUF60

UniProt Synonym Gene Names

FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1

NCBI Protein Information

poly(U)-binding-splicing factor PUF60

NCBI Summary for PUF60

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

UniProt Comments for PUF60

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cell junction; cyclin-dependent protein kinase activating kinase holoenzyme complex; intracellular ribonucleoprotein complex; nucleoplasm

Molecular Function: cadherin binding; DNA binding; identical protein binding; protein binding; RNA binding

Biological Process: apoptosis; mRNA splicing, via spliceosome; regulation of transcription, DNA-templated; transcription, DNA-dependent

Disease: Verheij Syndrome

Research Articles on PUF60

1. These results confirm that PUF60 is a major driver for the developmental, craniofacial, skeletal and cardiac phenotypes associated with the 8q24.3 microdeletion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PUF60 elisa kit

Reference Product	PubMed Publications
SF3B1 elisa kit	>2 publications with PUF60 and SF3B1
ERCC3 elisa kit	>1 publications with PUF60 and ERCC3
MYC elisa kit	>1 publications with PUF60 and MYC

Pathways associated with PUF60 elisa kit

Products by Pathway	Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram

Diseases associated with PUF60 elisa kit

Disease Name	Pubmed Publications
Ovarian Neoplasms Antibodies	>1 publications with PUF60 and Ovarian Neoplasms
Ovarian Diseases Antibodies	>1 publications with PUF60 and Ovarian Diseases
Neoplasm Metastasis Antibodies	>1 publications with PUF60 and Neoplasm Metastasis

Organs/Tissues associated with PUF60 elisa kit

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>1 publications with PUF60 and Heart
Stomach Antibodies	>1 publications with PUF60 and Stomach
Muscle Antibodies	>1 publications with PUF60 and Muscle
Lymph Antibodies	>1 publications with PUF60 and Lymph
Lymph Node Antibodies	>1 publications with PUF60 and Lymph Node
Intestine Antibodies	>1 publications with PUF60 and Intestine
Ovary Antibodies	>1 publications with PUF60 and Ovary
Skin Antibodies	>1 publications with PUF60 and Skin