Probable E3 Ubiquitin-Protein Ligase HERC1 (HERC1) ELISA Kit for Human

Name: Human HERC1 ELISA Kit; Probable E3 Ubiquitin-Protein Ligase HERC1; MyBioSource
Brand: MyBioSource
SKU: MBS280560
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280560

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HERC1 in samples. An antibody specific for HERC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHERC1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable E3 ubiquitin-protein ligase HERC1, ELISA Kit

Full Product Name

Human Probable E3 ubiquitin-protein ligase HERC1 (HERC1) ELISA Kit

Product Gene Name

HERC1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human HERC1. No significant cross-reactivity or interference between Human HERC1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

532,228 Da

OMIM

U50078 mRNA

3D Structure

ModBase 3D Structure for Q15751

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HERC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HERC1 purchase

MBS280560 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable E3 ubiquitin-protein ligase HERC1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HERC1. The ELISA analytical biochemical technique of the MBS280560 kit is based on HERC1 antibody-HERC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HERC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HERC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HERC1. It may not necessarily be applicable to this product.

NCBI GI #

126131099

NCBI GeneID

8925

NCBI Accession #

NP_003913.3

UniProt Primary Accession #

Q15751

UniProt Secondary Accession #

Q8IW65

UniProt Related Accession #

Q15751

GenBank Nucleotide #

NM_003922.3

NCBI Official Full Name

probable E3 ubiquitin-protein ligase HERC1

NCBI Official Synonym Full Names

HECT and RLD domain containing E3 ubiquitin protein ligase family member 1

NCBI Official Symbol

HERC1

NCBI Official Synonym Symbols

p532; p619; MDFPMR

UniProt Protein Name

Probable E3 ubiquitin-protein ligase HERC1

UniProt Synonym Protein Names

HECT domain and RCC1-like domain-containing protein 1; HECT-type E3 ubiquitin transferase HERC1; p532; p619

Protein Family

Probable E3 ubiquitin-protein ligase

UniProt Gene Name

HERC1

NCBI Protein Information

probable E3 ubiquitin-protein ligase HERC1

NCBI Summary for HERC1

This gen encodes a member of the HERC protein family. This protein stimulates guanine nucleotide exchange on ARF1 and Rab proteins. This protein may be involved in membrane transport processes. [provided by RefSeq, Mar 2012]

UniProt Comments for HERC1

HERC1: Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin- protein ligase which accepts ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

Protein type: EC 6.3.2.-; EC 6.3.2.19; GEF; GEF, ARF; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytoplasm; cytosol; Golgi apparatus; membrane

Molecular Function: ARF guanyl-nucleotide exchange factor activity; ubiquitin-protein transferase activity

Biological Process: cerebellar Purkinje cell differentiation; negative regulation of autophagy; neuromuscular process controlling balance; neuron projection development; positive regulation of GTPase activity; protein ubiquitination; transport

Disease: Macrocephaly, Dysmorphic Facies, And Psychomotor Retardation

Research Articles on HERC1

1. In the light of recent reports of HERC1 mutations in two families with a similar phenotypic presentation, this report reiterates the pathogenic nature and clinical consequences of HERC1 disruption.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HERC1 elisa kit

Reference Product	PubMed Publications
ARF1 elisa kit	>3 publications with HERC1 and ARF1
PKM2 elisa kit	>1 publications with HERC1 and PKM2
TP53 elisa kit	>1 publications with HERC1 and TP53

Pathways associated with HERC1 elisa kit

Products by Pathway	Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with HERC1 elisa kit

Disease Name	Pubmed Publications
Abnormalities, Multiple Antibodies	>2 publications with HERC1 and Abnormalities, Multiple
Carcinoma Antibodies	>2 publications with HERC1 and Carcinoma
Carcinoma, Squamous Cell Antibodies	>1 publications with HERC1 and Carcinoma, Squamous Cell
Breast Neoplasms Antibodies	>1 publications with HERC1 and Breast Neoplasms
Skin Neoplasms Antibodies	>1 publications with HERC1 and Skin Neoplasms
Immune System Diseases Antibodies	>1 publications with HERC1 and Immune System Diseases
Cell Transformation, Neoplastic Antibodies	>1 publications with HERC1 and Cell Transformation, Neoplastic
Kidney Diseases Antibodies	>1 publications with HERC1 and Kidney Diseases

Organs/Tissues associated with HERC1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>4 publications with HERC1 and Blood
Brain Antibodies	>3 publications with HERC1 and Brain
Nerve Antibodies	>2 publications with HERC1 and Nerve
Kidney Antibodies	>1 publications with HERC1 and Kidney
Intestine Antibodies	>1 publications with HERC1 and Intestine