Pre-Mrna-Splicing Factor SLU7 (SLU7) ELISA Kit for Human

Name: Human SLU7 ELISA Kit; Pre-Mrna-Splicing Factor SLU7; MyBioSource
Brand: MyBioSource
SKU: MBS280596
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280596

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLU7 in samples. An antibody specific for SLU7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLU7 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Pre-mRNA-splicing factor SLU7, ELISA Kit

Full Product Name

Human Pre-mRNA-splicing factor SLU7 (SLU7) ELISA Kit

Product Gene Name

SLU7 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human SLU7. No significant cross-reactivity or interference between Human SLU7 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

68,387 Da

OMIM

AF101074 mRNA

3D Structure

ModBase 3D Structure for O95391

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SLU7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLU7 purchase

MBS280596 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pre-mRNA-splicing factor SLU7, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLU7. The ELISA analytical biochemical technique of the MBS280596 kit is based on SLU7 antibody-SLU7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLU7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLU7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SLU7. It may not necessarily be applicable to this product.

NCBI GI #

27477111

NCBI GeneID

10569

NCBI Accession #

NP_006416.3

UniProt Primary Accession #

O95391

UniProt Secondary Accession #

Q3LUJ0; Q3LUJ1; Q6RXQ5; Q96FM9; D3DQK2

UniProt Related Accession #

O95391

GenBank Nucleotide #

NM_006425.4

NCBI Official Full Name

pre-mRNA-splicing factor SLU7

NCBI Official Synonym Full Names

SLU7 homolog, splicing factor

NCBI Official Symbol

SLU7

NCBI Official Synonym Symbols

9G8; hSlu7

UniProt Protein Name

Pre-mRNA-splicing factor SLU7

Protein Family

Pre-mRNA-splicing factor

UniProt Gene Name

SLU7

UniProt Synonym Gene Names

hSlu7

NCBI Protein Information

pre-mRNA-splicing factor SLU7

NCBI Summary for SLU7

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq, Jul 2008]

UniProt Comments for SLU7

SLU7: Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'- splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation. Belongs to the SLU7 family.

Protein type: RNA processing; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; membrane; nuclear speck; nucleoplasm; nucleus; small nuclear ribonucleoprotein complex; spliceosomal complex

Molecular Function: pre-mRNA 3'-splice site binding; protein binding; second spliceosomal transesterification activity; zinc ion binding

Biological Process: alternative mRNA splicing, via spliceosome; intracellular protein transport; mRNA 3'-end processing; mRNA export from nucleus; mRNA splicing, via spliceosome; nuclear mRNA 3'-splice site recognition; RNA export from nucleus; RNA splicing, via transesterification reactions; termination of RNA polymerase II transcription

Research Articles on SLU7

1. these findings indicate that SLU7 is co-opted by hepatocellular carcinoma (HCC) cells and other tumor cell types to maintain survival, and identify this splicing regulator as a new determinant for the expression of the oncogenic miR-17-92 cluster. This novel mechanism may be exploited for the development of antitumoral strategies in cancers displaying such SLU7-miR-17-92 crosstalk

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SLU7 elisa kit

Products by Pathway	Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram

Diseases associated with SLU7 elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>1 publications with SLU7 and Liver Diseases
Carcinoma Antibodies	>1 publications with SLU7 and Carcinoma
Immune System Diseases Antibodies	>1 publications with SLU7 and Immune System Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with SLU7 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with SLU7 and Liver Neoplasms
Inflammation Antibodies	>1 publications with SLU7 and Inflammation
Neoplasms Antibodies	>1 publications with SLU7 and Neoplasms

Organs/Tissues associated with SLU7 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with SLU7 and Blood
Liver Antibodies	>1 publications with SLU7 and Liver
Embryonic Tissue Antibodies	>1 publications with SLU7 and Embryonic Tissue