Poly (A) RNA Polymerase GLD2 (PAPD4) ELISA Kit for Human

Name: Human PAPD4 ELISA Kit; Poly (A) RNA Polymerase GLD2; MyBioSource
Brand: MyBioSource
SKU: MBS280598
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280598

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PAPD4 in samples. An antibody specific for PAPD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAPD4 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Poly (A) RNA polymerase GLD2 (PAPD4), ELISA Kit

Full Product Name

Human Poly (A) RNA polymerase GLD2 (PAPD4) ELISA Kit

Product Gene Name

PAPD4 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PAPD4. No significant cross-reactivity or interference between Human PAPD4 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

55,502 Da

OMIM

AK095818 mRNA

3D Structure

ModBase 3D Structure for Q6PIY7

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PAPD4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAPD4 purchase

MBS280598 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Poly (A) RNA polymerase GLD2 (PAPD4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PAPD4. The ELISA analytical biochemical technique of the MBS280598 kit is based on PAPD4 antibody-PAPD4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAPD4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAPD4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PAPD4. It may not necessarily be applicable to this product.

NCBI GI #

167555097

NCBI GeneID

167153

NCBI Accession #

NP_001107865.1

UniProt Primary Accession #

Q6PIY7

UniProt Secondary Accession #

Q86WZ2; Q8N927

UniProt Related Accession #

Q6PIY7

GenBank Nucleotide #

NM_001114393.2

NCBI Official Full Name

poly(A) RNA polymerase GLD2 isoform 1

NCBI Official Synonym Full Names

poly(A) RNA polymerase D4, non-canonical

NCBI Official Symbol

PAPD4

NCBI Official Synonym Symbols

GLD2; TUT2

UniProt Protein Name

Poly(A) RNA polymerase GLD2

UniProt Synonym Protein Names

PAP-associated domain-containing protein 4; Terminal uridylyltransferase 2; TUTase 2

Protein Family

Poly(A) RNA polymerase

UniProt Gene Name

PAPD4

UniProt Synonym Gene Names

GLD2; hGLD-2; TUTase 2

NCBI Protein Information

poly(A) RNA polymerase GLD2

UniProt Comments for PAPD4

PAPD4: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family. GLD2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.19; RNA processing; Transferase

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytosol

Molecular Function: ATP binding; metal ion binding; polynucleotide adenylyltransferase activity; protein binding

Biological Process: hemopoietic progenitor cell differentiation; hippocampus development; mRNA processing; neuron differentiation; retina development in camera-type eye; RNA polyadenylation

Research Articles on PAPD4

1. our results propose a novel feedback regulatory mechanism for controlling HCV RNA abundance by the viral capsid protein, which is capable of modulating miR-122 activity and stability through the inhibition of GLD-2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with PAPD4 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>2 publications with PAPD4 and Blood
Vascular Antibodies	>1 publications with PAPD4 and Vascular
Embryonic Tissue Antibodies	>1 publications with PAPD4 and Embryonic Tissue