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Y+l Amino Acid Transporter 1 (SLC7A7) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280633

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLC7A7 in samples. An antibody specific for SLC7A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A7 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Y+L amino acid transporter 1 (SLC7A7), ELISA Kit
Full Product Name
Mouse Y+L amino acid transporter 1 (SLC7A7) ELISA Kit
Product Gene Name
SLC7A7 elisa kit
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SLC7A7. No significant cross-reactivity or interference between Mouse SLC7A7 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
55,677 Da
3D Structure
ModBase 3D Structure for Q9Z1K8
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SLC7A7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC7A7 purchase

MBS280633 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Y+L amino acid transporter 1 (SLC7A7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC7A7. The ELISA analytical biochemical technique of the MBS280633 kit is based on SLC7A7 antibody-SLC7A7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC7A7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC7A7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SLC7A7. It may not necessarily be applicable to this product.

NCBI GI #
358439451
NCBI GeneID
20540
NCBI Accession #
NP_001240608.1
UniProt Primary Accession #
Q9Z1K8
UniProt Secondary Accession #
Q9QWS1
UniProt Related Accession #
Q9Z1K8
GenBank Nucleotide #
NM_001253679.1
NCBI Official Full Name
Y+L amino acid transporter 1
NCBI Official Synonym Full Names
solute carrier family 7 (cationic amino acid transporter, y+ system), member 7
NCBI Official Symbol
Slc7a7
NCBI Official Synonym Symbols
my+lat1; AI790233
UniProt Protein Name
Y+L amino acid transporter 1
UniProt Synonym Protein Names
Solute carrier family 7 member 7; y(+)L-type amino acid transporter 1; Y+LAT1; y+LAT-1
Protein Family
Y+L amino acid transporter
UniProt Gene Name
Slc7a7
UniProt Synonym Gene Names
Y+LAT1; y+LAT-1
NCBI Protein Information
Y+L amino acid transporter 1

UniProt Comments for SLC7A7

SLC7A7: Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Involved in the transport of L- arginine in monocytes. Defects in SLC7A7 are the cause of lysinuric protein intolerance (LPI). LPI is an autosomal recessive multisystem disorder found mainly in Finland and Italy. On a normal diet, LPI patients present poor feeding, vomiting, diarrhea, episodes of hyperammoniaemic coma and growth retardation. Hepatosplenomegaly, osteoporosis and a life- threatening pulmonary involvement (alveolar proteinosis) are also seen. Biochemically LPI is characterized by a defect in the plasma membrane transport of dibasic amino acids. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 14|14 C2

Cellular Component: integral component of membrane; integral component of plasma membrane; membrane; plasma membrane

Molecular Function: amino acid transmembrane transporter activity; antiporter activity; basic amino acid transmembrane transporter activity; L-amino acid transmembrane transporter activity

Biological Process: amino acid transport; regulation of arginine metabolic process; transport
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Research Articles on SLC7A7

1. The expression of Mct8 and L-type amino acid transporters Lat2 and Lat1 are determined in brain neurons during development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SLC7A7 elisa kit

 Products by Pathway  Pathway Diagram
 Amino Acid Transport Across The Plasma Membrane Pathway antibodies  Amino Acid Transport Across The Plasma Membrane Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram

Diseases associated with SLC7A7 elisa kit

 Disease Name  Pubmed Publications
 LYSINURIC PROTEIN INTOLERANCE Antibodies  >39 publications with SLC7A7 and LYSINURIC PROTEIN INTOLERANCE
 Amino Acid Metabolism, Inborn Errors Antibodies  >22 publications with SLC7A7 and Amino Acid Metabolism, Inborn Errors
 Kidney Diseases Antibodies  >8 publications with SLC7A7 and Kidney Diseases
 Neoplasms Antibodies  >5 publications with SLC7A7 and Neoplasms
 Coma Antibodies  >4 publications with SLC7A7 and Coma
 Lung Diseases Antibodies  >4 publications with SLC7A7 and Lung Diseases
 Vomiting Antibodies  >3 publications with SLC7A7 and Vomiting
 Lung Neoplasms Antibodies  >2 publications with SLC7A7 and Lung Neoplasms
 Anemia Antibodies  >2 publications with SLC7A7 and Anemia
 Adenocarcinoma Antibodies  >2 publications with SLC7A7 and Adenocarcinoma

Organs/Tissues associated with SLC7A7 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >17 publications with SLC7A7 and Intestine
 Kidney Antibodies  >16 publications with SLC7A7 and Kidney
 Blood Antibodies  >13 publications with SLC7A7 and Blood
 Lung Antibodies  >6 publications with SLC7A7 and Lung
 Brain Antibodies  >4 publications with SLC7A7 and Brain
 Bone Antibodies  >3 publications with SLC7A7 and Bone
 Embryonic Tissue Antibodies  >3 publications with SLC7A7 and Embryonic Tissue
 Bone Marrow Antibodies  >1 publications with SLC7A7 and Bone Marrow
 Testis Antibodies  >1 publications with SLC7A7 and Testis
 Mammary Gland Antibodies  >1 publications with SLC7A7 and Mammary Gland
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