Human PLEKHA8 ELISA Kit – Cat# MBS280660

Pleckstrin Homology Domain-Containing Family A Member 8 (PLEKHA8) ELISA Kit for Human

Name: Human PLEKHA8 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS280660
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280660

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLEKHA8 in samples. An antibody specific for PLEKHA8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLEKHA8 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Pleckstrin homology domain-containing family A member 8 (PLEKHA8), ELISA Kit

Full Product Name

Human Pleckstrin homology domain-containing family A member 8 (PLEKHA8) ELISA Kit

Product Gene Name

PLEKHA8 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PLEKHA8. No significant cross-reactivity or interference between Human PLEKHA8 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

49,462 Da

OMIM

AF380162 mRNA

3D Structure

ModBase 3D Structure for Q96JA3

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PLEKHA8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLEKHA8 purchase

MBS280660 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pleckstrin homology domain-containing family A member 8 (PLEKHA8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLEKHA8. The ELISA analytical biochemical technique of the MBS280660 kit is based on PLEKHA8 antibody-PLEKHA8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLEKHA8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLEKHA8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PLEKHA8. It may not necessarily be applicable to this product.

NCBI GI #

308153327

NCBI GeneID

84725

NCBI Accession #

NP_001183955.1

UniProt Primary Accession #

Q96JA3

UniProt Secondary Accession #

Q7Z5V8; Q9BU78; Q9H274; Q9H8Z7; B4DH00

UniProt Related Accession #

Q96JA3

GenBank Nucleotide #

NM_001197026.1

NCBI Official Full Name

pleckstrin homology domain-containing family A member 8 isoform 1

NCBI Official Synonym Full Names

pleckstrin homology domain containing A8

NCBI Official Symbol

PLEKHA8

NCBI Official Synonym Symbols

FAPP2

UniProt Protein Name

Pleckstrin homology domain-containing family A member 8

UniProt Synonym Protein Names

Phosphatidylinositol-four-phosphate adapter protein 2; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2; hFAPP2; Serologically defined breast cancer antigen NY-BR-86

Protein Family

Pleckstrin homology domain-containing family

UniProt Gene Name

PLEKHA8

UniProt Synonym Gene Names

FAPP2; PH domain-containing family A member 8; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2; hFAPP2

NCBI Protein Information

pleckstrin homology domain-containing family A member 8

UniProt Comments for PLEKHA8

PLEKHA8: Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans- Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: Golgi apparatus; Golgi membrane; nucleoplasm; trans-Golgi network

Molecular Function: glycolipid binding; glycolipid transporter activity

Biological Process: glycolipid transport; lipid transport; phosphatidylinositol biosynthetic process; protein transport

Research Articles on PLEKHA8

1. Altogether, this study implies that hepatitis C virus coopts FAPP2 for virus genome replication via phosphatidylinositol 4-phosphate binding and glycosphingolipid transport to the hepatitis C virus replication complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PLEKHA8 elisa kit

Reference Product	PubMed Publications
ARF1 elisa kit	>1 publications with PLEKHA8 and ARF1

Diseases associated with PLEKHA8 elisa kit

Disease Name	Pubmed Publications
Chromosome Aberrations Antibodies	>1 publications with PLEKHA8 and Chromosome Aberrations

Organs/Tissues associated with PLEKHA8 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>2 publications with PLEKHA8 and Kidney
Heart Antibodies	>1 publications with PLEKHA8 and Heart
Brain Antibodies	>1 publications with PLEKHA8 and Brain