Potassium Intermediate/Small Conductance Calcium-Activated Channel (KCNN2) ELISA Kit for Human

Name: Human KCNN2 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS280663
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280663

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNN2 in samples. An antibody specific for KCNN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNN2 present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Potassium intermediate/small conductance calcium-activated channel (KCNN2), ELISA Kit

Full Product Name

Human Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2) ELISA Kit

Product Gene Name

KCNN2 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human KCNN2. No significant cross-reactivity or interference between Human KCNN2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

26,341 Da

OMIM

AF239613 mRNA

3D Structure

ModBase 3D Structure for Q9H2S1

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of KCNN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KCNN2 purchase

MBS280663 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Potassium intermediate/small conductance calcium-activated channel (KCNN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KCNN2. The ELISA analytical biochemical technique of the MBS280663 kit is based on KCNN2 antibody-KCNN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KCNN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KCNN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KCNN2. It may not necessarily be applicable to this product.

NCBI GI #

501353609

NCBI GeneID

3781

NCBI Accession #

NP_001265133.1

UniProt Primary Accession #

Q9H2S1

UniProt Secondary Accession #

Q0VFZ4; Q6PJI0; Q6X2Y2; A6NF94

UniProt Related Accession #

Q9H2S1

GenBank Nucleotide #

NM_001278204.1

NCBI Official Full Name

small conductance calcium-activated potassium channel protein 2 isoform b

NCBI Official Synonym Full Names

potassium calcium-activated channel subfamily N member 2

NCBI Official Symbol

KCNN2

NCBI Official Synonym Symbols

SK2; hSK2; SKCA2; KCa2.2; SKCa 2

UniProt Protein Name

Small conductance calcium-activated potassium channel protein 2

UniProt Synonym Protein Names

KCa2.2

Protein Family

Small conductance calcium-activated potassium channel protein

UniProt Gene Name

KCNN2

UniProt Synonym Gene Names

SK2; SKCa 2; SKCa2

NCBI Protein Information

small conductance calcium-activated potassium channel protein 2

NCBI Summary for KCNN2

Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene is a member of the KCNN family of potassium channel genes. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

UniProt Comments for KCNN2

KCNN2: Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin. Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q22.3

Cellular Component: cell soma; cell surface; dendritic spine; integral component of membrane; plasma membrane; Z disc

Molecular Function: alpha-actinin binding; calcium-activated potassium channel activity; calmodulin binding; protein binding; protein domain specific binding; protein homodimerization activity; small conductance calcium-activated potassium channel activity

Biological Process: potassium ion transport

Research Articles on KCNN2

1. Study establishes the distribution profile of SK2 channel protein in human brain.The expression of SK2 human isoform b in brain could explain the variability of electrophysiological findings observed with SK2 channels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KCNN2 elisa kit

Reference Product	PubMed Publications
KCNN3 elisa kit	>38 publications with KCNN2 and KCNN3
KCNA3 elisa kit	>2 publications with KCNN2 and KCNA3
ACTN2 elisa kit	>2 publications with KCNN2 and ACTN2
KCNMA1 elisa kit	>2 publications with KCNN2 and KCNMA1

Pathways associated with KCNN2 elisa kit

Products by Pathway	Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway antibodies	Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway Diagram
Ca2+ Activated K+ Channels Pathway antibodies	Ca2+ Activated K+ Channels Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Potassium Channels Pathway antibodies	Potassium Channels Pathway Diagram
Serotonergic Synapse Pathway antibodies	Serotonergic Synapse Pathway Diagram

Diseases associated with KCNN2 elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>16 publications with KCNN2 and Heart Diseases
Arrhythmias, Cardiac Antibodies	>11 publications with KCNN2 and Arrhythmias, Cardiac
Disease Models, Animal Antibodies	>7 publications with KCNN2 and Disease Models, Animal
Memory Disorders Antibodies	>5 publications with KCNN2 and Memory Disorders
Neurobehavioral Manifestations Antibodies	>4 publications with KCNN2 and Neurobehavioral Manifestations
Inflammation Antibodies	>3 publications with KCNN2 and Inflammation
Mental Disorders Antibodies	>2 publications with KCNN2 and Mental Disorders
Pain Antibodies	>2 publications with KCNN2 and Pain
Learning Disorders Antibodies	>2 publications with KCNN2 and Learning Disorders
Seizures Antibodies	>2 publications with KCNN2 and Seizures

Organs/Tissues associated with KCNN2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>56 publications with KCNN2 and Brain