Human POLDIP3 ELISA Kit – Cat# MBS280774

Polymerase Delta-Interacting Protein 3 (POLDIP3) ELISA Kit for Human

Name: Human POLDIP3 ELISA Kit; Polymerase Delta-Interacting Protein 3; MyBioSource
Brand: MyBioSource
SKU: MBS280774
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280774

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate POLDIP3 in samples. An antibody specific for POLDIP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLDIP3 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Polymerase delta-interacting protein 3 (POLDIP3), ELISA Kit

Full Product Name

Human Polymerase delta-interacting protein 3 (POLDIP3) ELISA Kit

Product Gene Name

POLDIP3 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human POLDIP3. No significant cross-reactivity or interference between Human POLDIP3 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

42,895 Da

OMIM

AY422990 Genomic DNA

3D Structure

ModBase 3D Structure for Q9BY77

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of POLDIP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLDIP3 purchase

MBS280774 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Polymerase delta-interacting protein 3 (POLDIP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing POLDIP3. The ELISA analytical biochemical technique of the MBS280774 kit is based on POLDIP3 antibody-POLDIP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POLDIP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POLDIP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for POLDIP3. It may not necessarily be applicable to this product.

NCBI GI #

29837655

NCBI GeneID

84271

NCBI Accession #

NP_115687.2

UniProt Primary Accession #

Q9BY77

UniProt Secondary Accession #

Q009A7; Q5H972; Q6PGN6; Q7Z6Z0; Q9NSP5; Q9NSP6; A8K6F8; A8K6V9

UniProt Related Accession #

Q9BY77

GenBank Nucleotide #

NM_032311.4

NCBI Official Full Name

polymerase delta-interacting protein 3 isoform 1

NCBI Official Synonym Full Names

DNA polymerase delta interacting protein 3

NCBI Official Symbol

POLDIP3

NCBI Official Synonym Symbols

SKAR; PDIP3; PDIP46

UniProt Protein Name

Polymerase delta-interacting protein 3

UniProt Synonym Protein Names

46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAR

Protein Family

Polymerase delta-interacting protein

UniProt Gene Name

POLDIP3

UniProt Synonym Gene Names

KIAA1649; PDIP46; p46; SKAR

NCBI Protein Information

polymerase delta-interacting protein 3

NCBI Summary for POLDIP3

This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

UniProt Comments for POLDIP3

SKAR: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cytoplasm; cytoplasmic ribonucleoprotein granule; cytosol; nuclear speck; nucleoplasm; transcription export complex

Molecular Function: protein binding; RNA binding

Biological Process: mRNA 3'-end processing; mRNA export from nucleus; poly(A)+ mRNA export from nucleus; positive regulation of translation; RNA export from nucleus; termination of RNA polymerase II transcription

Research Articles on POLDIP3

1. Altered expression of PDIP46 or its mutation may affect Pol delta functions in vivo, and thereby be a nexus for altered genomic stability.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with POLDIP3 elisa kit

Products by Pathway	Pathway Diagram
MTOR Signaling Pathway antibodies	MTOR Signaling Pathway Diagram

Diseases associated with POLDIP3 elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>2 publications with POLDIP3 and Nervous System Diseases
Death Antibodies	>1 publications with POLDIP3 and Death
Inflammation Antibodies	>1 publications with POLDIP3 and Inflammation
Kidney Diseases Antibodies	>1 publications with POLDIP3 and Kidney Diseases

Organs/Tissues associated with POLDIP3 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with POLDIP3 and Brain
Blood Antibodies	>2 publications with POLDIP3 and Blood
Vascular Antibodies	>1 publications with POLDIP3 and Vascular
Kidney Antibodies	>1 publications with POLDIP3 and Kidney
Lung Antibodies	>1 publications with POLDIP3 and Lung