Protein Wnt-2 (WNT2) ELISA Kit for Human

Name: Human WNT2 ELISA Kit; Protein Wnt-2; MyBioSource
Brand: MyBioSource
SKU: MBS280778
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280778

Intended Uses: For the quantitative detection of Human Protein Wnt-11 (WNT11) concentration in serum, plasma, cell culture supernates and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WNT11 in Human… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Protein Wnt-2 (WNT2), ELISA Kit

Full Product Name

Human Protein Wnt-2 (WNT2) ELISA Kit

Product Gene Name

WNT2 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, Plasma, Cell Culture Supernates And Other Biological Fluids

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

15.6 pg/mL (mean of 6 independent assays).

Specificity

This assay has high sensitivity and excellent specificity for detection of Human WNT11. No significant cross-reactivity or interference between Human WNT11 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

40,418 Da

OMIM

X07876 mRNA

3D Structure

ModBase 3D Structure for P09544

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of WNT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNT2 purchase

MBS280778 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Wnt-2 (WNT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT2. The ELISA analytical biochemical technique of the MBS280778 kit is based on WNT2 antibody-WNT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WNT2. It may not necessarily be applicable to this product.

NCBI GI #

4507927

NCBI GeneID

7472

NCBI Accession #

NP_003382.1

UniProt Primary Accession #

P09544

UniProt Secondary Accession #

Q75N05; Q9UDP9; A4D0V1

UniProt Related Accession #

P09544

GenBank Nucleotide #

NM_003391.2

NCBI Official Full Name

protein Wnt-2

NCBI Official Synonym Full Names

Wnt family member 2

NCBI Official Symbol

WNT2

NCBI Official Synonym Symbols

IRP; INT1L1

UniProt Protein Name

Protein Wnt-2

UniProt Synonym Protein Names

Int-1-like protein 1; Int-1-related protein; IRP

Protein Family

Protein

UniProt Gene Name

WNT2

UniProt Synonym Gene Names

INT1L1; IRP; IRP

NCBI Protein Information

protein Wnt-2

NCBI Summary for WNT2

This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for WNT2

WNT2: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Cell adhesion; Cell development/differentiation; Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; frizzled binding; protein binding; receptor ligand activity

Biological Process: atrial cardiac muscle morphogenesis; cell fate commitment; cell proliferation in midbrain; cell-cell signaling; lens development in camera-type eye; lung development; neurogenesis; neuron differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of neurogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; Wnt signaling pathway

Research Articles on WNT2

1. Three-dimensional organotypic co-culture assays revealed that WNT2-mediated fibroblast motility and extracellular matrix remodeling enhanced cancer cell invasion of cell lines even harboring mutations in the adenomatous polyposis coli/beta-catenin pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with WNT2 elisa kit

Products by Pathway	Pathway Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
Basal Cell Carcinoma Pathway antibodies	Basal Cell Carcinoma Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
DNA Damage Response (only ATM Dependent) Pathway antibodies	DNA Damage Response (only ATM Dependent) Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
Hedgehog Signaling Pathway antibodies	Hedgehog Signaling Pathway Diagram
Hedgehog Signaling Pathway antibodies	Hedgehog Signaling Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram

Diseases associated with WNT2 elisa kit

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>21 publications with WNT2 and Disease Models, Animal
Breast Neoplasms Antibodies	>19 publications with WNT2 and Breast Neoplasms
Adenocarcinoma Antibodies	>19 publications with WNT2 and Adenocarcinoma
Fibrosis Antibodies	>15 publications with WNT2 and Fibrosis
Lung Diseases Antibodies	>13 publications with WNT2 and Lung Diseases
Cell Transformation, Neoplastic Antibodies	>13 publications with WNT2 and Cell Transformation, Neoplastic
Neoplasm Metastasis Antibodies	>12 publications with WNT2 and Neoplasm Metastasis
Autistic Disorder Antibodies	>12 publications with WNT2 and Autistic Disorder
Lung Neoplasms Antibodies	>11 publications with WNT2 and Lung Neoplasms
Neoplasm Invasiveness Antibodies	>6 publications with WNT2 and Neoplasm Invasiveness

Organs/Tissues associated with WNT2 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>47 publications with WNT2 and Lung
Embryonic Tissue Antibodies	>41 publications with WNT2 and Embryonic Tissue
Brain Antibodies	>26 publications with WNT2 and Brain
Placenta Antibodies	>14 publications with WNT2 and Placenta
Intestine Antibodies	>13 publications with WNT2 and Intestine
Kidney Antibodies	>10 publications with WNT2 and Kidney
Uterus Antibodies	>9 publications with WNT2 and Uterus
Connective Tissue Antibodies	>7 publications with WNT2 and Connective Tissue
Prostate Antibodies	>6 publications with WNT2 and Prostate
Testis Antibodies	>6 publications with WNT2 and Testis