Doublesex- and Mab-3-Related Transcription Factor 1 (DMRT1) ELISA Kit for Pig

DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes, and is found to be the key sex-determining factor in chickens. This gene exhibits a gonad-specific and sexually dimorphic expression pattern, just like the related doublesex gene in fruit flies. The protein contains a nuclear localization signal between the 2 zinc-binding sites of the DM domain, which is located in the N terminus. Immunohistochemical analysis of adult human testis revealed that DMRT1 localized to nuclei of Sertoli cells, spermatogonia, and spermatocytes.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DMRT1 in samples. An antibody specific for DMRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DMRT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280789 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DMRT1. The ELISA analytical biochemical technique of the MBS280789 kit is based on DMRT1 antibody-DMRT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DMRT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DMRT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).