Zinc Finger Homeobox Protein 3 (ZFHX3) ELISA Kit for Mouse

Tissue-specific expression of the human alpha-fetoprotein (AFP) gene is strongly stimulated by an enhancer present 3.3 to 4.9 kb upstream of the transcription initiation site. To determine the nuclear factor in hepatoma cell lines that interacts with the human AFP enhancer AT motif, Morinaga et al. (1991) screened a hepatoma cDNA expression library with an AFP enhancer fragment that bore the AT motif. They succeeded in isolating a cDNA that can code for an AT motif-binding factor, which they termed ATBF1. This was the largest DNA-binding protein identified to that time and the first protein shown to contain multiple homeodomains and multiple zinc finger motifs. The protein had a predicted mass of 306 kD and contained 4 homeodomains and 17 zinc finger motifs.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFHX3 in samples. An antibody specific for ZFHX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFHX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFHX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFHX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ZFHX3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280797 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger homeobox protein 3 (ZFHX3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZFHX3. The ELISA analytical biochemical technique of the MBS280797 kit is based on ZFHX3 antibody-ZFHX3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZFHX3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZFHX3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).