Probable E3 Ubiquitin-Protein Ligase TRIM8 (TRIM8) ELISA Kit for Human

Name: Human TRIM8 ELISA Kit; Probable E3 Ubiquitin-Protein Ligase TRIM8; MyBioSource
Brand: MyBioSource
SKU: MBS280802
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280802

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TRIM8 in Human serum, plasma. An antibody specific for TRIM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIM8 present is… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable E3 ubiquitin-protein ligase TRIM8, ELISA Kit

Full Product Name

Human Probable E3 ubiquitin-protein ligase TRIM8 (TRIM8) ELISA Kit

Product Gene Name

TRIM8 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

0.156 ng/mL - 10 ng/mL

Sensitivity

0.1 ng/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human TRIM8. No significant cross-reactivity or interference between Human TRIM8 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

61,489 Da

OMIM

AF281046 mRNA

3D Structure

ModBase 3D Structure for Q9BZR9

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TRIM8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIM8 purchase

MBS280802 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable E3 ubiquitin-protein ligase TRIM8, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRIM8. The ELISA analytical biochemical technique of the MBS280802 kit is based on TRIM8 antibody-TRIM8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRIM8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRIM8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM8. It may not necessarily be applicable to this product.

NCBI GI #

148596968

NCBI GeneID

81603

NCBI Accession #

NP_112174.2

UniProt Primary Accession #

Q9BZR9

UniProt Secondary Accession #

Q9C028; A6NI31

UniProt Related Accession #

Q9BZR9

GenBank Nucleotide #

NM_030912.2

NCBI Official Full Name

probable E3 ubiquitin-protein ligase TRIM8 isoform 1

NCBI Official Synonym Full Names

tripartite motif containing 8

NCBI Official Symbol

TRIM8

NCBI Official Synonym Symbols

GERP; RNF27

UniProt Protein Name

Probable E3 ubiquitin-protein ligase TRIM8

UniProt Synonym Protein Names

Glioblastoma-expressed RING finger protein; RING finger protein 27; RING-type E3 ubiquitin transferase TRIM8Curated; Tripartite motif-containing protein 8

Protein Family

Probable E3 ubiquitin-protein ligase

UniProt Gene Name

TRIM8

UniProt Synonym Gene Names

GERP; RNF27

NCBI Protein Information

probable E3 ubiquitin-protein ligase TRIM8

NCBI Summary for TRIM8

This gene encodes a member of the tripartite motif (TRIM) protein family. Based on similarities to other proteins, the encoded protein is suspected to be an E3 ubiquitin-protein ligase. Regulation of this gene may be altered in some cancers. Mutations resulting in a truncated protein product have been observed in early-onset epileptic encephalopathy (EOEE). [provided by RefSeq, Sep 2016]

UniProt Comments for TRIM8

TRIM8: Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. Belongs to the TRIM/RBCC family.

Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: cytosol; PML body

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transferase activity; zinc ion binding

Biological Process: innate immune response; negative regulation of viral transcription; negative regulation of virion penetration into host cell; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of NF-kappaB transcription factor activity; positive regulation of transcription factor activity; protein ubiquitination; stem cell maintenance

Research Articles on TRIM8

1. TRIM8 activates STAT3 by suppressing the expression of PIAS3, an inhibitor of STAT3, most likely through E3-mediated ubiquitination and proteasomal degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TRIM8 elisa kit

Reference Product	PubMed Publications
PIAS3 elisa kit	>2 publications with TRIM8 and PIAS3
SOCS1 elisa kit	>1 publications with TRIM8 and SOCS1

Pathways associated with TRIM8 elisa kit

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interferon Signaling Pathway antibodies	Interferon Signaling Pathway Diagram
Interferon Gamma Signaling Pathway antibodies	Interferon Gamma Signaling Pathway Diagram

Diseases associated with TRIM8 elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>2 publications with TRIM8 and Necrosis
Inflammation Antibodies	>1 publications with TRIM8 and Inflammation

Organs/Tissues associated with TRIM8 elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>2 publications with TRIM8 and Embryonic Tissue
Blood Antibodies	>1 publications with TRIM8 and Blood
Placenta Antibodies	>1 publications with TRIM8 and Placenta
Larynx Antibodies	>1 publications with TRIM8 and Larynx
Brain Antibodies	>1 publications with TRIM8 and Brain
Kidney Antibodies	>1 publications with TRIM8 and Kidney
Lung Antibodies	>1 publications with TRIM8 and Lung