Probable ATP-Dependent RNA Helicase DDX4 (DDX4) ELISA Kit for Human

Name: Human DDX4 ELISA Kit; Probable ATP-Dependent RNA Helicase DDX4; MyBioSource
Brand: MyBioSource
SKU: MBS280921
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280921

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDX4 in samples. An antibody specific for DDX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX4 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable ATP-dependent RNA helicase DDX4, ELISA Kit

Full Product Name

Human Probable ATP-dependent RNA helicase DDX4 (DDX4) ELISA Kit

Product Gene Name

DDX4 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human DDX4. No significant cross-reactivity or interference between Human DDX4 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

77,056 Da

OMIM

AY004154 mRNA

3D Structure

ModBase 3D Structure for Q9NQI0

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DDX4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DDX4 purchase

MBS280921 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable ATP-dependent RNA helicase DDX4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDX4. The ELISA analytical biochemical technique of the MBS280921 kit is based on DDX4 antibody-DDX4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDX4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDX4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DDX4. It may not necessarily be applicable to this product.

NCBI GI #

216548263

NCBI GeneID

54514

NCBI Accession #

NP_001136021.1

UniProt Primary Accession #

Q9NQI0

UniProt Secondary Accession #

Q5M7Z3; Q86VX0; Q9NT92; Q9NYB1; A8K8Q2; B3KSF4; D6RDK4; E9PCD8

UniProt Related Accession #

Q9NQI0

GenBank Nucleotide #

NM_001142549.1

NCBI Official Full Name

probable ATP-dependent RNA helicase DDX4 isoform 2

NCBI Official Synonym Full Names

DEAD-box helicase 4

NCBI Official Symbol

DDX4

NCBI Official Synonym Symbols

VASA

UniProt Protein Name

Probable ATP-dependent RNA helicase DDX4

UniProt Synonym Protein Names

DEAD box protein 4; Vasa homolog

Protein Family

Probable ATP-dependent RNA helicase

UniProt Gene Name

DDX4

UniProt Synonym Gene Names

VASA

NCBI Protein Information

probable ATP-dependent RNA helicase DDX4

NCBI Summary for DDX4

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

UniProt Comments for DDX4

DDX4: May play a role in germ cell development. May play a role in sperm motility. Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. Belongs to the DEAD box helicase family. DDX4/VASA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; nucleolus; perinuclear region of cytoplasm

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; ATPase activity; nucleic acid binding

Biological Process: cell differentiation; DNA methylation during gametogenesis; male meiosis I; male meiotic nuclear division; multicellular organism development; RNA secondary structure unwinding; RNA-mediated gene silencing; sperm motility; spermatogenesis

Research Articles on DDX4

1. Results suggest that DDX4 positively regulates cell cycle progression of diverse somatic-derived blood cancer cells, implying its broad contributions to the cancer cell phenotype.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DDX4 elisa kit

Reference Product	PubMed Publications
PIWIL2 elisa kit	>6 publications with DDX4 and PIWIL2
SPSB1 elisa kit	>1 publications with DDX4 and SPSB1

Diseases associated with DDX4 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6 publications with DDX4 and Neoplasms
Congenital Abnormalities Antibodies	>1 publications with DDX4 and Congenital Abnormalities
Pain Antibodies	>1 publications with DDX4 and Pain
Urogenital Abnormalities Antibodies	>1 publications with DDX4 and Urogenital Abnormalities
Infertility, Female Antibodies	>1 publications with DDX4 and Infertility, Female

Organs/Tissues associated with DDX4 elisa kit

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>42 publications with DDX4 and Testis
Mixed Antibodies	>2 publications with DDX4 and Mixed
Brain Antibodies	>1 publications with DDX4 and Brain